卵形鲳鲹应对流速胁迫的代谢组学分析

Metabolomic analysis of Trachinotus ovatus under flow velocity stress

流速是深远海养殖的关键环境因子之一。为了深入解析卵形鲳鲹在流速胁迫下的分子调控机制,分别以静水环境、中等流速(54 cm/s)和高流速(90 cm/s)水流对卵形鲳鲹进行胁迫,利用LC/MS的代谢组学技术探究肝脏内源性代谢物的变化,寻找差异代谢物以及相关的代谢通路。结果表明,与静水组相比,中流速组有51种代谢产物的含量发生显著变化,富集在40条通路中,主要包括半乳糖代谢(galactose metabolism)、糖异生(gluconeogenesis)、不饱和脂肪酸的合成(biosynthesis of unsaturated fatty acids)、花生四烯酸代谢(arachidonic acid metabolism)等;高流速组有40种代谢产物的含量发生显著变化,富集在22条通路中,主要包括饱和脂肪酸的合成(biosynthesis of unsaturated fatty acids)、泛酸和辅酶A的生物合成(pantothenate and CoA biosynthesis)、赖氨酸降解(lysine degradation)、花生四烯酸代谢(arachidonic acid metabolism)、亚油酸代谢(linoleic acid metabolism)等。与中流速组相比,高流速组有31种代谢产物含量发生显著变化,富集在12条通路中,主要包括花生四烯酸代谢(arachidonic acid metabolism)、脂肪细胞因子信号通路(adipocytokine signaling pathway)、半胱氨酸和蛋氨酸代谢(cysteine and methionine metabolism)等。通路分析表明中、高流速胁迫均促进了不饱和脂肪酸代谢、花生四烯酸代谢和泛酸的合成,鱼体通过加强代谢来提高游泳能力,中流速组主要显著影响了半乳糖代谢、半胱氨酸和蛋氨酸的代谢,卵形鲳鲹此时产生了更多能量用于运动;高流速组则显著影响了L-赖氨酸、维甲酸的代谢,揭示了在面对高流速胁迫时,鱼体的游泳能力在下降,部分免疫机能触发。

Flow velocity is one of the key environmental factors for deep sea aquaculture.In order to further under-stand the molecular regulation mechanism of golden pompano(T.ovatus)under flow velocity stress,the fishes were stressed under the static water,medium flow velocity(54 cm/s)and high flow velocity(90 cm/s),and the LC/MS metabolomics technology was used to explore the changes of endogenous metabolites in the liver to look for differential metabolites and related metabolic pathways.The results show that 51 metabolites in the medium flow velocity group have significant difference with which in the static water and are enriched in 40 pathways,mainly including galactose metabolism,gluconeogenesis,biosynthesis of unsaturated fatty acids,arachidonic acid metabolism,etc.Forty metabolites in the high flow velocity group have significant difference with which in the stat-ic water and are enriched in 22 pathways,mainly including biosynthesis of unsaturated fatty acids,pantothenate and CoA biosynthesis,lysine degradation,arachidonic acid metabolism,linoleic acid metabolism,etc.Compared with the medium flow velocity group,31 metabolites in the high flow velocity group have significant difference and are enriched in 12 pathways,mainly including arachidonic acid metabolism,adipocytokine signaling pathway,cysteine and methionine metabolism,etc.Pathway analysis shows that both medium and high flow velocity stress promoted unsaturated fatty acid metabolism,arachidonic acid metabolism and pantothenic acid synthesis.The ability of swim-ming is improved to against the stress of flow velocity by strengthening metabolism.The medium flow velocity group mainly significantly affected the metabolism of galactose,cysteine and methionine.At this time,more and more energy produced by fish is used for exercise.The high flow velocity group mainly significantly affect the metabolism of L-lysine and retinoic acid,which reveals that the swimming ability of fish may be decreased and some immune functions may be triggered under the high flow velocity stress.