马铃薯HAK基因家族鉴定及耐盐性表达分析

Identification of Potato HAK Gene Family and Analysis of Salt Tolerance Expression

高亲和性K+转运蛋白(high-affinity K+transporter,HAK)是植物中重要的K+转运蛋白家族之一,参与植物的多种生命过程,特别是K+的吸收和转运.马铃薯高质量参考基因组的公布,为HAK基因家族成员的全面鉴定和分析提供了重要支撑.本次研究从DM8.1中共鉴定出24个HAK家族成员,这些家族成员的氨基酸残基数目为249~1617个,分子量为27252.70~181106.69 U,等电点为5.92~9.35,其蛋白产物均定位在细胞质膜上.盐处理后,StHAK23的表达量在根部和叶片同时显著上调;StHAK2、StHAK4、StHAK10和StHAK14只在叶片中特异地显著上调;StHAK7、StHAK8、StHAK11、StHAK13和StHAK22只在根部特异性表达;剩余成员在根部或叶片中低水平表达或不表达.研究结果表明HAK基因家族的不同成员在响应盐胁迫的过程中发挥着不同的功能.

High-affinity K+transporter(HAK)is one of the most important K+transporter families in plants,and is involved in many life processes,especially K+absorption and transport.The publication of the high-quality reference genome of potato provides an important support for the comprehensive identification and analysis of HAK gene family members.In this study,a total of 24 HAK family members were identified from DM8.1,with 249~1617 amino acid residues,molecular weights of 27252.70~181106.69 U,isoelectric points of 5.92~9.35,and their protein products were located on the plasma membrane of the cell.After salt treatment,the expression levels of StHAK23 was significantly up-regulated in roots and leaves.StHAK2、StHAK4、StHAK10 and StHAK14 were significantly up-regulated only in the leaves.StHAK7、StHAK8、StHAK11、StHAK13 and StHAK22 were significantly up-regulated only in the roots.The remaining members were expressed at low or no level in roots or leaves.The results indicate that different members of the HAK gene family play different functions in response to salt stress.