绞股蓝总苷对急性期缺血性脑卒中小鼠NF-κB及TNF-α表达的影响

Effect of gypenosides on expression of NF-κB and TNF-αin mice with acute ischemic stroke

目的探讨绞股蓝总苷对急性期缺血性脑卒中小鼠核因子-κB(NF-κB)及肿瘤坏死因子-α(TNF-α)表达的影响。方法将24只健康雄性C57小鼠随机分为假手术组、缺血性脑卒中组、绞股蓝总苷低剂量组、绞股蓝总苷高剂量组,每组6只。除假手术组外,其余组小鼠采用Longa线栓法阻塞大脑中动脉建立缺血性脑卒中模型。术后2 h,绞股蓝总苷低、高剂量组分别给予绞股蓝总苷100 mg/kg、200 mg/kg腹腔注射,假手术组和缺血性脑卒中组均给予等量生理盐水腹腔注射。术后24 h对各组小鼠进行神经功能缺损评分,使用TTC染色测定各组小鼠脑梗死体积,分别使用Western blot法和qRT-PCR法检测手术组、缺血性脑卒中组、绞股蓝总苷高剂量组小鼠脑组织中NF-κB、TNF-α蛋白及mRNA表达情况。结果绞股蓝总苷低剂量组小鼠的神经功能缺损评分与缺血性脑卒中组比较差异无统计学意义(P>0.05),脑梗死体积占比明显低于缺血性脑卒中组(P<0.05);绞股蓝总苷高剂量组小鼠的神经功能缺损评分和脑梗死体积占比均明显低于缺血性脑卒中组和绞股蓝总苷低剂量组(P均<0.05)。缺血性脑卒中组小鼠脑组织中NF-κB、TNF-α蛋白及mRNA表达量均明显高于假手术组(P均<0.05),绞股蓝总苷高剂量组小鼠脑组织中NF-κB、TNF-α蛋白及mRNA表达量均明显低于缺血性脑卒中组(P均<0.05)。结论在缺血性脑卒中急性期,绞股蓝总苷可通过下调NF-κB、TNF-α表达,减轻炎症反应,减小脑梗死体积,保护脑组织。

Objective It is to investigate the effect of gypenosides on the expression of nuclear factor-κB(NF-κB)and tumor necrosis factor-α(TNF-α)in mice with acute ischemic stroke.Methods Twenty-four healthy male C57 mice were randomly divided into sham operation group,ischemic stroke group,gypenosides low dose group,and gypenosides high dose group,with 6 mice in each group.Th ischemic stroke models were established by blocking the middle cerebral artery using the Longa wire embolization method in the mice of all groups except for the sham operation group.At 2 hours after operation,the mice in the gypenosides low-dose group and gypenosides high-dose group were injected intraperitoneally with gypenosides 100 mg/kg and 200 mg/kg respectively,the mice in the sham operation group and ischemic stroke group were given equal volume of normal saline by intraperitoneal injection.At 24 h after surgery,the scores of neurological deficits of the mice in each group were scored,and the volume of cerebral infarction of the mice in each group was measured by TTC staining,the expressions of NF-κB and TNF-αproteins of the mice in each group were detected by Western blot,and the expressions of NF-κB and TNF-αmRNA of the mice in each group were detected by qRT-PCR.Results The difference in the neurological deficit scores of mice between the gypenosides low-dose group and ischemic stroke group was not statistically significant(P>0.05),and the percentage of cerebral infarction volume was significantly lower than that in the ischemic stroke group(P<0.05);the neurological deficit scores and the percentage of cerebral infarction volume of mice in the gypenosides high-dose group were significantly lower than those in the ischemic stroke group and the gypenosides low-dose group(all P<0.05).The expressions of NF-κB,TNF-αprotein and mRNA in the brain tissues of mice in the ischemic stroke group were significantly higher than those in the sham operation group(all P<0.05),and the expressions of NF-κB,TNF-αprotein and mRNA in the brain tissues of mice in the gypenosides high-dose group were significantly lower than those in the ischemic stroke group(all P<0.05).Conclusion Gypenosides could down-regulate the expression of NF-κB and TNF-α,alleviate the inflammatory response and reduce the infarct volume to protect the brain tissue in the acute stage of ischemic stroke.