单细胞测序技术在动脉粥样硬化研究的应用进展

Application progress of single cell transcriptome sequencing in the study of atherosclerosis

单细胞测序技术作为近10年来高速发展起来的一门新兴技术,能够在单个细胞水平上对基因组、转录组、表观遗传组、蛋白组甚至多组学联合进行高通量测序,以揭示细胞的异质性,并通过分析获得不同组织器官细胞的基因表达谱,从而帮助我们更加深入理解各个组织器官的细胞多样性。动脉粥样硬化是许多心血管疾病病理的主要原因,其所引起的并发症如心肌梗死和卒中是导致全球性死亡的重要原因。近年来,单细胞测序技术已被广泛地应用于动脉粥样硬化的研究,并取得了重要进展。本文主要就单细胞测序技术在动脉粥样硬化研究中的最新应用进展作一综述。

Single-cell sequencing technology is an emerging technology that has developed rapidly in the past decade. It can perform high-throughput sequencing of the genome, transcriptome, epigenome, proteome and even multi-omics at the single cell level to reveal cellular heterogeneity. By analyzing the gene expression profiles of cells in different tissues and organs, it helps us to better understand the cellular diversity of various tissues and organs. Atherosclerosis is the main cause of pathology of many cardiovascular diseases, and its complications such as myocardial infarction and stroke are important causes of global death. In recent years, single-cell sequencing technology has been widely used in the study of atherosclerosis and has made important progress. This article mainly reviews the latest application progress of single-cell sequencing technology in the study of atherosclerosis.