土牛膝醇提物提取工艺优化及体外抗人肺腺癌A549细胞活性研究

Optimization of extraction technology of alcohol extract from Achyranthes aspera L.and its activity against human lung adenocarcinoma A549 cells in vitro

土牛膝是应用广泛的药用植物,其功效众多,为研究其醇提物抗癌功效,采用Box-Behnken响应面法优化土牛膝的醇提工艺,并对醇提物进行体外人肺腺癌A549细胞毒性及细胞划痕试验。获得了最佳提取工艺为:提取温度90℃,液料比40∶1,提取时间2 h,在此条件下,土牛膝醇提物提取率为9.23%。细胞毒性试验结果显示,随着醇提物浓度的上升,A549细胞的存活率迅速下降,当醇提物浓度为256μg/mL时,对A549细胞的抑制率达94.65%,且细胞划痕结果显示醇提物对A549细胞迁移有一定抑制作用,可为研究土牛膝药用特性提供参考。

Achyranthes aspera L.is a widely used medicinal plant with many effects.In order to study the anti-cancer effect of its alcohol extract,the alcohol extraction process from A.aspera was optimized by Box-Behnken response surface method,and the cytotoxicity and cell scratch test of human lung cancer A549 cells were carried out in vitro.The optimum extraction conditions were as follows:extraction temperature 90℃,liquid-solid ratio 40∶1,extraction time 2 h.Under these conditions,the extraction rate of alcohol extract from A.aspera was 9.23%.The results of cytotoxicity test showed that the survival rate of A549 cells decreased rapidly with the increase of alcohol extract concentration.When the concentration of alcohol extract was 256μg/mL,the inhibition rate of A549 cells was 94.65%.The cell scratch results showed that alcohol extract had a certain inhibitory effect on the migration of A549 cells,which can provide a reference for the study of the medicinal properties from A.aspera.