单细胞转录组测序在糖尿病视网膜病变中的应用

Application of Single-Cell RNA Sequencing in Diabetic Retinopathy

糖尿病视网膜病变(DR)是致工作人群眼盲的首位病因,发病机制错综复杂,尚未阐明。单细胞转录组测序(scRNA-seq)通过深入分析细胞异质性、功能变化及相互作用,为在单细胞分辨率上探讨DR发病机制提供了有力工具。现笔者对新兴的scRNA-seq技术及其在DR中的应用,包括揭示DR的细胞类型和基因表达特征、解析DR发病机制、挖掘新的诊疗靶点和生物标志物的研究等进行综述。

Diabetic retinopathy(DR)is the leading cause of blindness in the working population,and the pathogenesis is intricate and yet to be elucidated.Single-cell RNA-seq(scRNA-seq)provides a powerful tool to explore the pathogenesis of DR at single-cell resolution through in-depth analysis of cellular heterogeneity,functional changes and interactions.This article presents a review of the emerging scRNA-seq technology and its applications in DR,including studies of revealing the cell types and gene expression characteristics,deciphering the pathogenesis,and exploring new therapeutic targets and biomarkers of DR.