摘要
R-loops are chromatin structures consisting of anRNA:DNA hybrid and the other single-stranded DNA,which widely exist among genomes from bacteria to higher eukaryotes and participate in a variety of biological processes(Zhou et al.,2022).Currently,a variety of approaches to detect genome-wide R-loops have been developed,and ssDRIP-seq(single-strand DNA ligation-based library preparation from DNA:RNA hybrid immunoprecipitation,followed by sequencing)is one of the widely utilized methods(Xu et al.,2022).However,there are many limitations to genome-wide R-loop mapping based on high-throughput methods.For example,the activity of restriction enzymes for genomic DNA fragmentation.
基金
supported by the Digitalization,Development,and Application of Biotic Resource Project(202002AA100007 to C.Z.)
the National Natural Science Foundation of China(grant nos.31822028 and 91940306 to Q.S.and 32100428 to J.Z.)
and the Ministry of Science and Technology of the People’s Republic of China(2016YFA0500800 to Q.S.).We greatly appreciate the useful discussions by all the members from the Zhang lab and the Sun lab.The Zhang Lab is supported by the Yunnan Young&Elite Talents Project(YNWR-QNBJ-2019-268).J.Z.is supported by the postdoctoral fellowship from Tsinghua-Peking Center for Life Sciences.
