生信分析Top2a在胃癌中的表达和功能富集

Expression and functional enrichment of Top2a in gastric cancer based on bioinformatics analysis

[目的]通过生物信息学分析了解Top2a在胃癌组织中的表达和功能富集。[方法]GEO数据库获取胃癌芯片,筛选生物钟基因,String数据库及Cytoscape软件绘制蛋白互作图(PPI),富集分析确定基因生物学意义,表达差异分析、ROC曲线绘制及生存分析确定最终的关键基因(hub-gene),GSEA分析挖掘hub-gene涉及通路,TISCH数据库确定基因与胃癌肿瘤微环境(TME)的关系。[结果]从GSE79973和GSE65801获取300个DEGs,其中有3个生物钟基因——Top2a、Ghrl、Nrob2。PPI图获得与3个生物钟基因存在相互作用关系的53个基因,GO联合KEGG分析发现,其功能及通路主要富集在物质吸收与分泌、细胞粘附、细胞间质成分构建等。表达差异分析、生存预后分析、临床诊断价值分析获得最终hub-gene——Top2a,GSEA富集结果发现Top2a涉及细胞增殖、基因调控、基体锚定、表观遗传调控等通路,TME相关分析发现Top2a的表达与细胞间质及粘液分泌有着密切关联。[结论]生物钟基因Top2a是胃癌的独立不良预后因素,参与包括细胞分裂过程、癌基因调控以及细胞间质转变等过程。

[Objective]Understanding the expression and functional enrichment of Top2a in gastric cancer by bioinformatics analysis.[Method]Acquisition of gastric cancer microarrays using GEO database to screen for circadian clock genes,string database and cytoscape software to obtain protein-protein interaction,enrichment analysis to determine biological significance of the genes,GSEA analysis to mine hub-gene involved pathways,TISCH database analysis of the relationship between genes and the tumor microenvironment(TME)in gastric cancer.[Result]300 DEGs were obtained from GSE79973 and GSE65801 with 3 circadian clock genes-Top2a,Ghrl,Nrob2.53 genes with interaction with the 3 biological clock genes were obtained from PPI map,GO+KEGG analysis find their functions and pathways were enriched in substance uptake and secretion,cell adhesion,and intercellular component construction.Analysis of expression differences,survival prognosis analysis,and clinical diagnostic value analysis yielded the final hub-gene,Top2a.GSEA enrichment of Top2a revealed pathways mostly involved in cell proliferation,gene regulation,matrix anchoring,epigenetic regulation,etc.The final TME correlation analysis revealed the final TME correlation analysis revealed that the expression of Top2a was closely associated with interstitial cell and mucus secretion.[Conclusion]The circadian clock gene Top2a is an independent poor prognostic factor in gastric cancer,involving mechanisms such as cell division processes,gene regulation,and intercellular transformation.