摘要
Reading biomolecular signatures and understanding their role in health and disease is one of the greatest scientific challenges in modern biology.Decoding this information is not only foundational for biology but also a cornerstone for next generation molecular diagnostics.This calls for novel methods that can capture the presence and identity of low-abundance compounds at the individual molecule level.Since its inception in the 1980s,nanopore sequencing has become an essential part of the single-molecule sensing toolkit,proving that long,labelfree reads of DNA can be achieved at low cost and high throughput.1 Despite their huge success in genome sequencing,reading the linear sequence of proteins is a considerably more complex task that requires differentiating 20 different amino acids(as opposed to the 4 DNA bases),as well as their modifications,during real-time translocation.Deconvoluting the time-dependent electrical current traces to determine entire amino acid sequences over long reads is as yet an unaccomplished milestone.
基金
funding from the European Union’s Horizon 2020 research and innovation program(ERC StG,SIMPHONICS,Project No.101041486)
X.Y.acknowledges funding from the Chinese Scholarship Council(Scholarship No.202108270002).All authors acknowledge K.Watanabe and T.Taniguchi from the National Institute of Materials Science(NIMS)for the bulk hBN crystals.
