湖羊SRP68基因多态性及其与生长性状的关联分析

Polymorphism of SRP 68 Gene and Its Association with Growth Traits in Hu Sheep

【目的】研究信号识别颗粒68(signal recognition particle 68,SRP68)基因在不同月龄湖羊不同组织中的表达情况,并分析其多态性对湖羊生长性状的影响,以期找到与绵羊生长性状显著相关的分子标记。【方法】利用实时荧光定量PCR检测SRP 68基因在湖羊心脏、肝脏、脾脏、肺脏、肾脏和背最长肌6种组织中的表达情况,以及在初生、45日龄、4月龄和6月龄时湖羊背最长肌中的表达情况;采用绵羊Illumina OvineSNP50 BeadChip对3024只湖羊SRP 68基因多态位点进行基因型检测;使用SPSS 26.0软件对SRP 68基因多态位点与1974只湖羊生长性状进行关联分析。【结果】SRP 68基因在湖羊不同组织中均有表达,在45日龄和4月龄背最长肌、心脏中的表达量极显著高于其他组织(P<0.01),在6月龄背最长肌中的表达量极显著高于其他组织(P<0.01)。SRP 68基因rs421715384 G>A位点在湖羊群体中处于中度多态性(0.25<PIC<0.5),AA和GG基因型个体初生重和2月龄体重均显著高于GA基因型(P<0.05),AA基因型个体5月龄体高显著高于GA和GG基因型(P<0.05),GG基因型个体6月龄体重、管围和胸宽均显著高于AA基因型(P<0.05)。【结论】SRP 68基因与湖羊肌肉发育密切相关,rs421715384 G>A位点可作为湖羊生长性状的潜在分子标记,为湖羊分子育种提供参考。

【Objective】This experiment was aimed to investigate the expression of signal recognition particle 68(SRP68)gene of different tissues in Hu sheep at different months,and analyze the effect of its polymorphism on the growth traits in Hu sheep,in order to find the molecular markers significantly related to the growth traits in sheep.【Method】The expression of SRP 68 gene in heart,liver,spleen,lung,kidney and longissimus dorsi muscle of Hu sheep was detected by Real-time quantitative PCR,as well as in the longissimus dorsi muscle of Hu sheep at birth,45-day,4-month-old and 6-month-old.The polymorphism site of SRP 68 gene was detected by Illumina OvineSNP 50 BeadChip in 3024 Hu sheep.The association between polymorphism site of SRP 68 gene and the growth traits of 1974 Hu sheep was analyzed by SPSS 26.0 software.【Result】SRP 68 gene was expressed in different tissues of Hu sheep,and the expression of longissimus dorsi muscle and heart in Hu sheep at 45-day and 4-month-old were extremely significantly higher than that of other tissues(P<0.01),and the expression of longissimus dorsi muscle at 6-month-old was extremely significantly higher than that of other tissues(P<0.01).There was moderate polymorphism at rs421715384 G>A of SRP 68 gene in Hu sheep population(0.25<PIC<0.5).The birth weight and 2-month-old weight of AA and GG genotypes were significantly higher than that of GA genotype(P<0.05).The 5-month-old body height of AA genotype was significantly higher than that of GA and GG genotypes(P<0.05),and the 6-month-old body weight,tube circumference and chest width of GG genotype were significantly higher than that of AA genotype(P<0.05).【Conclusion】SRP 68 gene was closely related to the muscle development of Hu sheep,and rs421715384 G>A could be used as a potential molecular marker for growth traits in Hu sheep,which could provide a reference for molecular breeding in Hu sheep.