杭州地区宠物衣原体流行病学调查

Epidemiological Investigation of Chlamydia in Pets in Hangzhou

【目的】了解杭州地区宠物衣原体的流行情况。【方法】采用PCR和间接血凝试验(IHA)对杭州某动物医院238份宠物犬眼结膜拭子、556份宠物猫眼结膜拭子、98份宠物鸟血清样本和花鸟市场264份鹦鹉血清样本进行衣原体检测,并对阳性样本进行核苷酸序列测定。利用SPSS 25.0软件分析动物的年龄、性别对衣原体感染有无统计学意义;利用Clustal W和Mega 11.0软件将流行株的主要毒力基因与已发表的参考序列进行相似性比对、进化关系分析。【结果】与宠物猫相比,动物医院中宠物犬和鸟不易感染衣原体,宠物猫检测出阳性样本13份,阳性率为2.34%。幼猫(<1岁)的感染率(3.57%)最高,公猫的感染率(2.70%)略高于母猫(2.02%),差异均不显著(P>0.05)。13份猫阳性样本测序分析显示,均为猫衣原体(Chlamydia felis),与猫衣原体Fe/C-56 ompA基因相似性在99.2%~99.7%之间。花鸟市场鹦鹉检测出阳性样本14份,阳性率为5.30%。受感染的鹦鹉中幼鸟(<1岁)的感染率(9.78%)最高,雌鸟感染率(6.25%)高于雄鸟(4.41%),差异均不显著(P>0.05)。花鸟市场的鹦鹉感染率(5.30%)高于动物医院的鸟(0),14份鹦鹉阳性样本测序分析显示,均为鹦鹉热衣原体(Chlamydia psittaci),与鹦鹉热衣原体6BC ompA基因相似性在99.4%~99.5%之间。【结论】本研究首次对杭州地区宠物衣原体流行情况进行调查,结果表明杭州市宠物猫和花鸟市场鹦鹉存在衣原体的感染,给人类健康带来了潜在威胁,因此,应当提高对宠物衣原体感染的重视,采取适当的饲养管理措施防控衣原体感染,避免人兽共患。

【Objective】The aim of this study was to understand the prevalence of Chlamydia in pets in Hangzhou.【Method】238 pet dog conjunctival samples,556 pet cat conjunctival samples,98 pet bird serum samples and 264 parrot serum samples from a flower and bird market in Hangzhou were tested for Chlamydia using PCR and indirect hemagglutination assay(IHA),and the positive samples were sequenced.SPSS 25.0 software was used to analyze whether the age and gender of animals had statistical significance for Chlamydia infection.The similarity and evolutionary relationship between the main virulence genes of the epidemic strains and the published reference sequences were analyzed by Clustal W and Mega 11.0 softwares.【Result】The results showed that pet dogs and birds were less susceptible to Chlamydia infection in animal hospitals compared to pet cats,and 13 positive samples were detected for pet cats,with a positive rate of 2.34%.The infection rate(3.57%)was highest in young cats(<1 year old)and slightly higher in male cats(2.70%)than in female cats(2.02%),all differences were not significant(P>0.05).Sequencing analysis of 13 positive cat samples revealed that all were Chlamydia felis,with genetic similarity to Chlamydia felis Fe/C-56 ompA gene ranging from 99.2%to 99.7%.14 positive samples were detected in parrots from flower and bird markets,with a positive rate of 5.30%.The infection rate(9.78%)was highest among infected parrots in young birds(<1 year old)and higher in females(6.25%)than in males(4.41%),all differences were not statistically significant(P>0.05).The prevalence of infection in parrots from flower and bird markets(5.30%)was higher than that in birds from animal hospitals(0),and 14 positive samples from parrots were sequenced and analyzed and found to be Chlamydia psittaci,with genetic similarity to Chlamydia psittaci 6BC ompA gene ranging from 99.4%to 99.5%.【Conclusion】In this study,the prevalence of Chlamydia in pets in Hangzhou was investigated for the first time,and the results showed that Chlamydia infection existed in pet cats and parrots in flower and bird markets in Hangzhou,posing a potential threat to human health.Therefore,the importance of Chlamydia infection in pets should be raised and appropriate feeding management measures should be taken to prevent and control Chlamydia infection and avoid human-animal co-infection.