摘要
【目的】了解拥有养殖-屠宰-运输-销售环节的现代化农牧食品企业鸡肉生产链各环节中沙门氏菌耐药情况及分子分型特征。【方法】本研究对在2020年10月-2021年09月从苏北某农牧食品企业下属的养殖场、屠宰厂、运输车辆、专营店分季度连续采集样品中分离得到的37株沙门氏菌,采用纸片扩散法进行13种常用抗菌药物敏感试验,PCR进行11种耐药基因检测、脉冲场凝胶电泳(PFGE)进行分子分型检测。【结果】37株沙门氏菌均耐药,对氨苄西林耐药率最高,为67.57%,对多西环素、庆大霉素、四环素耐药率超过50%,对头孢西丁耐药率最低,为8.11%,各环节沙门氏菌分离菌株对氨苄西林的耐药率均超过50%,沿该鸡肉生产链氨苄西林耐药率呈现下降趋势,耐药率同样呈现下降趋势的抗菌药有头孢他啶、头孢曲松、环丙沙星、四环素,耐药率呈现上升趋势的抗菌药有头孢西丁、甲氧苄氨嘧啶、庆大霉素、丁胺卡那霉素;耐3种及以上抗菌药的菌株为34株,多重耐药率为94.59%,养殖环节、运输环节和销售环节沙门氏菌分离株的多重耐药率均为100%;养殖环节沙门氏菌分离株耐药物种类数最多集中在耐7种及以上抗菌药,对8种抗菌药的耐药率高于其他环节;耐药基因中bla PSE的检出率最高,为56.76%,未检测到tetC耐药基因;PFGE分型共有26种不同的PFGE带型产生,相似度100%的带型有7组。【结论】苏北某农牧食品企业鸡肉生产链中沙门氏菌有较高的耐药性,多重耐药严重,养殖环节耐药性高于其他环节,PFGE分型提示耐药菌株能够沿生产链“养殖-屠宰”的途径传播,且长期存在,养殖环节避免耐药沙门菌的产生是保障鸡肉产品安全生产关键点。
【Objective】To understand the drug resistance and molecular typing characteristics of Salmonella in the chicken production chain of modern agricultural and animal husbandry food enterprises with the links of breeding,slaughtering,transportation and sales.【Method】In this study,37 strains of Salmonella isolated from the continuous samples collected from the farms,slaughterhouses,transport vehicles and franchised stores of an agricultural and animal husbandry food enterprise in Northern Jiangsu from October 2020 to September 2021 were tested for 13 common antibiotics by paper diffusion method,11 drug resistance genes by PCR,and molecular typing by pulsed field gel electrophoresis(PFGE).【Result】The 37 strains of Salmonella isolated were all resistant,the highest resistance rate to ampicillin was 67.57%,the resistance rate to doxycycline,gentamicin and tetracycline was more than 50%,and the lowest resistance rate to cefoxitin was 8.11%.The resistance rate of Salmonella isolated from all links to ampicillin was more than 50%.Along the chicken production chain,the resistance rate of ampicillin showed a downward trend,and the drug resistance rate of ceftazidime,ceftriaxone,ciprofloxacin and tetracycline also showed a downward trend.The antibiotic resistance rate of cefoxitin,trimethoprim,gentamicin,amikacin showed an upward trend.34 strains were resistant to three or more antibiotics,and the multidrug resistance rate was 94.59%.The multidrug resistance rate of Salmonella isolated in breeding,transportation and marketing was 100%.The number of drug-resistant strains of Salmonella isolated from the links of breeding was mostly concentrated in 7 or more antibiotics,and the drug resistance rate to 8 antibiotics was higher than that of other links.The detection rate of drug resistance gene bla PSE was the highest,and its detection rate was 56.76%.The drug resistance gene tetC was not detected.PFGE classification had produced 26 different PFGE belt types,and there were 7 groups of belt types with 100%similarity.【Conclusion】Salmonella isolated from the chicken production chain of a modern agricultural and animal husbandry food enterprise in Northern Jiangsu had high drug resistance and serious multidrug resistance,and the drug resistance in the breeding link was higher than that in other links.PFGE typing suggested that the drug resistant strains could spread along the“Breeding-slaughtering”route of the chicken production chain,and existed for a long time.Avoiding the production of drug resistant Salmonella in the breeding link was the key point to ensure the safe production of chicken products.
作者
王玉燕
张林吉
迟兰
陈永亮
任士飞
孙朋
WANG Yuyan;ZHANG Linji;CHI Lan;CHEN Yongliang;REN Shifei;SUN Peng(Xuzhou Vocational College of Bioengineering,Xuzhou 221006,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2023年第8期3345-3353,共9页
China Animal Husbandry & Veterinary Medicine
基金
江苏省高等学校自然科学研究项目(20KJB230009)
徐州市科技局项目(KC21297)
徐州市推动科技创新专项资金项目(KC20050)。