响应面优化超声辅助提取青椒叶多酚工艺及抗氧化研究

Optimization of ultrasonic-assisted extraction of polyphenols from green pepper leaves by response surface methodology and its antioxidant activity

以青椒叶为原料,采用响应面设计优化青椒叶多酚提取工艺,分析青椒叶多酚的抗氧化活性。以多酚的提取率为指标,在单因素试验基础上,利用Box-Behnken设计进行响应面试验,确定青椒叶多酚最佳提取工艺,并对多酚清除DPPH和OH自由基的能力进行分析。结果表明:青椒叶多酚最佳提取工艺为乙醇浓度71%、超声时间41 min,液料比25∶1(mL∶g)和提取温度71℃,在最佳工艺条件下得到多酚提取率为67.62 mg/g。与模型预测值相比,其相对误差仅为0.21%,证明了基于响应面分析方法优化青椒叶多酚提取工艺的有效性和可行性。青椒叶多酚能够有效地抵抗氧化作用,并且其抗氧化活性与多酚浓度呈正相关,对DPPH自由基和OH自由基清除率的半抑制质量浓度分别为56.34和125.20 mg/L,该研究为青椒叶多酚在保健品和食品工业等领域的应用提供了参考。

The extraction process of polyphenols from green pepper leaves was optimized by response surface methodology,using green pepper leaves as raw materials,and the antioxidant activity of polyphenols from green pepper leaves was analyzed.Taking the extraction yield of polyphenols as an index,on the basis of single factor experiment,response surface experiment was conducted by Box-Behnken design to determine the best extraction process of polyphenols from green pepper leaves,and the scavenging ability of polyphenols to DPPH and OH free radicals was analyzed.The results showed that the optimum extraction process of polyphenols from green pepper leaves was as follows:ethanol concentration 71%,ultrasonic time 41 min,liquid-solid ratio 25∶1(mL∶g)and extraction temperature 71℃.Under the optimized conditions,the extraction yield of polyphenols was 67.62 mg/g.Compared with the predicted value of the model,the relative error was only 0.21%,which proved the effectiveness and feasibility of optimizing the extraction process of polyphenols from green pepper leaves based on response surface analysis.Green pepper leaf polyphenols could effectively resist oxidation,and its antioxidant activity was positively correlated with polyphenol concentration.The semi-inhibitory mass concentration IC 50 of DPPH radical and OH radical scavenging rate was 56.34mg/L and 125.20 mg/L,respectively.This study provided a reliable scientific basis for the antioxidant capacity of green pepper leaf polyphenols,and provided a reference for the application of green pepper leaf polyphenolsin health care products and food industry.