兔抗新型冠状病毒血清制备中佐剂对抗体效价的影响

Effects of adjuvants in rabbit anti-SARS-CoV-2 serum preparation on the antibody titer

目的制备高效价兔抗新型冠状病毒血清并研究佐剂对抗体效价的影响.方法将9只新西兰兔随机分为a、b、c组,分别皮下注射新型冠状病毒混合弗氏佐剂、氢氧化铝佐剂或单磷酰脂A佐剂进行免疫,0、28、42 d各免疫1次.每次免疫前及初次免疫后14 d于耳中央动脉采血,初次免疫后49 d经颈动脉采血,分离血清,进行中和抗体效价检测以及中和试验.结果经过整个免疫程序后,a、b、c组均能产生高效价抗血清,且a组(6144.00)最终产生的血清中和抗体效价高于b组(4437.33)和c组(3754.67);3组血清均可在中和试验中有效中和KMS2株新型冠状病毒,中和后培养细胞均未发生病变.结论3种不同佐剂疫苗在新西兰兔体内均可产生高水平的免疫应答.

Objective To preparate high-efficacy rabbit anti-severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)serum and to study the effect of different adjuvants in rabbit anti-SARSCoV-2 serum preparation on the antibody titer.Methods Nine New Zealand rabbits were randomly divided into groups a,b,and c,and were immunized by subcutaneous injections with SARS-CoV-2 mixed with Freund's adjuvant,aluminum hydroxide adjuvant,or monophosphoryl lipid A adjuvant,respectively.They were immunized 3 times on 0,28 and 42 d.Blood samples were collected from the central ear artery before every immunization,and at 14 d after the first immunization.Blood was also collected from the carotid artery on 49 d after the first immunization to separate the serum for neutralizing antibody titer detection and neutralizing tests.Results After the entire immunization process,all groups produced highly effective anti-SARS-CoV-2 serum.Group a(6144.00)had higher final neutralizing antibody titer than groups b(4437.33)and c(3754.67).The serum obtained from all 3 groups could effectively neutralize the KMS2 strain of SARSCoV-2 in neutralizing tests without causing any cell damage.Conclusion All 3 different adjuvants can be used to obtain highly effective anti-SARS-CoV-2 serum in New Zealand rabbits.