西藏麻黄乙醇提取物及其不同极性组分的体外抗氧化活性研究

The in vitro antioxidant activities of the ethanol extract and its different polar fractions from Ephedra saxatilis Royle ex Florin in Tibet

本文对西藏麻黄乙醇提取物及其不同极性提取物的体外抗氧化活性进行了研究。首先采用化学显色方法对大类化学成分进行鉴别,然后分别采用Folin-Ciocalteu法和氯化铝比色法对总多酚和总黄酮的含量进行鉴定,再采用DPPH法、ABTS法、羟自由基清除和还原粉末法对其抗氧化活性进行了研究。麻黄中乙酸乙酯部分和二氯甲烷部分的抗氧化活性均优于BHT,这与麻黄中多酚和黄酮含量较高有关。

In the present study,we aimed to investigate the in vitro antioxidant activity of the ethanol extract and its polar extracts from Ephedra saxatilis Royle ex Florin in Tibet.The chemical constituents were first screened by the preliminary phytochemical methods.The contents of total polyphenols and total flavonoids were identified by the Folin-Ciocalteu method and colorimetric method of the aluminum chloride method,respectively.Furthermore,their antioxidant activities were investigated by the DPPH method,ABTS method,hydroxyl radical scavenging,and reduction powder method.The preliminary phytochemical screening showed that the polyphenols,glycosides,flavonoids,and saponins existed in ethanol extract,and the chemical composition in different polar extracts varied,among which the contents of polyphenols and flavonoids were highest in the ethyl acetate part,followed by the dichloromethane part,exhibiting best antioxidant capacity.Both the ethyl acetate and dichloromethane parts from Ephedra saxatilis Royle ex Florin exhibited better antioxidant activities than BHT,which was associated with the high contents of polyphenols and flavonoids.