红景天苷对近视豚鼠脉络膜厚度及HIF-1α和多巴胺及其受体表达的影响

Effect of salidroside on choroidal thickness and expression of hypoxia-inducible factor-1α,dopamine and its receptor in guinea pigs with myopia

目的:探讨红景天苷(SA)对透镜诱导型近视(LIM)豚鼠脉络膜厚度及缺氧诱导因子-1α(HIF-1α)、多巴胺及其D1受体表达的影响。方法:将18只2周龄豚鼠随机分为正常对照(NC)组、LIM组和透镜诱导型近视+红景天苷(LIM+SA)组,每组6只。NC组正常饲养,给予2mL/d生理盐水灌胃;LIM组豚鼠右眼前配戴-5D透镜,建立近视模型,给予2mL/d生理盐水灌胃;LIM+SA组豚鼠右眼前配戴-5D透镜同时给予2mL/d红景天苷(100mg/kg)灌胃。造模4wk后测量各组豚鼠右眼屈光度、眼轴长度及脉络膜厚度,通过实时荧光定量PCR(qPCR)和免疫组织化学(IHC)检测各组豚鼠右眼脉络膜视网膜HIF-1α mRNA及蛋白表达量,通过酶联免疫吸附实验(ELISA)和Western blot法检测多巴胺浓度及其D1受体表达量。结果:造模4wk后,与NC组比较,LIM组和LIM+SA组豚鼠右眼屈光度均负向增加,眼轴均延长,脉络膜厚度均减小,脉络膜视网膜HIF-1α mRNA和蛋白表达量均增加,多巴胺浓度及其D1受体表达量均降低;与LIM组比较,LIM+SA组豚鼠右眼近视屈光度明显减小,眼轴长度较短,脉络膜厚度增加,脉络膜视网膜HIF-1α mRNA和蛋白表达量均降低,多巴胺浓度及其D1受体表达量均增加。结论:红景天苷干预近视豚鼠可通过影响脉络膜厚度及HIF-1α、多巴胺及其D1受体的表达延缓近视进展。

AIM:To examine the effects of salidroside on choroidal thickness,hypoxia-inducible factor-1α(HIF-1α),dopamine(DA)and its D1 receptor expression in guinea pigs with lens-induced myopia(LIM).METHODS:A total of 18 two-week-old guinea pigs were randomly divided into the normal control(NC)group,the LIM group,and the LIM+salidroside(LIM+SA)group,with 6 guinea pigs in each group.The guinea pigs in the NC group were fed normally and intragastrically administered with 2 mL/d saline;those in the LIM group wore a-5D lens in front of their right eyes to establish a myopia model,then they were intragastrically administered with 2 mL/d saline.Finally,those in the LIM+SA group wore glasses along with intragastric administration of 2 mL/d salidroside at a dose of 100 mg/kg.The refraction,axial length,and choroidal thickness of guinea pigs in each group were measured 4wk following the establishment of the model.In addition,the relative mRNA expression and protein content of HIF-1αin the choroid and retina of guinea pigs in each group were detected by real-time quantitative PCR(qPCR)and immunohistochemistry(IHC).Finally,the DA concentration and its D1 receptor expression were detected by enzyme-linked immunosorbent assay(ELISA)and Western blot.RESULTS:At 4wk after model establishment,guinea pigs of LIM group and LIM+SA group exhibited increased negative refraction of the right eye,prolonged axial length,and decreased choroidal thickness compared to the NC group.The relative mRNA expression and protein content of HIF-1αin the choroid and retina of the guinea pigs increased.The concentration of DA and the expression of its D1 receptor both decreased.Moreover,compared to the LIM group,the diopter of the right eye of guinea pigs in LIM+SA group significantly reduced,the axial length was shorter,the thickness of choroid increased,the relative mRNA expression and protein content of HIF-1αin the choroid and retina decreased and the concentration of DA and the expression of its D1 receptor both increased.CONCLUSION:Salidroside can delay myopia progression in myopic guinea pigs by affecting choroidal thickness and the expression of HIF-1α,DA and its D1 receptor.