硒化甘草多糖对免疫抑制小鼠免疫功能的影响

Effect of selenide Glycyrrhiza polysaccharide on immune function of immunosuppressed mice

试验旨在探究硒化甘草多糖(SeGUP)对免疫抑制小鼠免疫功能的影响。选取96只昆明小鼠,雌、雄各半,随机分为6组,每组16只小鼠,利用环磷酰胺(CY)给除空白组外小鼠构建免疫低下的模型,腹腔注射CY 80 mg/g,连续3 d。第4 d开始空白组、模型组小鼠每天腹腔注射0.3 mL生理盐水,阳性组腹腔注射200 mg/g黄芪多糖(APS)混悬液,SeGUP高、中、低剂量组小鼠腹腔注射100、200、300 mg/g SeGUP混悬液,连续7 d。结果显示,SeGUP高、中剂量组小鼠体重极显著高于SeGUP低剂量组(P<0.01)。SeGUP高、中剂量组小鼠碳粒廓清指数(K)、吞噬指数(α)极显著高于SeGUP低剂量组(P<0.01)。SeGUP高剂量组小鼠血清白细胞介素-2 (IL-2)含量极显著高于SeGUP中剂量组、阳性组(P<0.01),SeGUP中剂量组、阳性组小鼠血清IL-2含量极显著高于SeGUP低剂量组、空白组(P<0.01),SeGUP高、中剂量组小鼠血清肿瘤坏死因子-α(TNF-α)、免疫球蛋白M (IgM)、干扰素-γ(IFN-γ)、免疫球蛋白G (IgG)含量极显著高于SeGUP低剂量组(P<0.01)。SeGUP高、中剂量组小鼠胸腺指数极显著高于SeGUP低剂量组(P<0.01)。研究表明,SeGUP可通过下调小鼠脾脏指数,上调体重、K、α、IL-2、TNF-α、IgM、IFN-γ、IgG、胸腺指数等指标,增强免疫抑制小鼠的免疫功能,其中200、300 mg/g的SeGUP效果较好。

The study was to investigate the effect of SeGUP on the immunomodulatory effect of immunosuppressed mice.A total of 96 Kunming mice,half female and half male,were randomly divided into six groups with 16 mice in each group.Mice was given CY to establish an immunocompromised model except the blank group,and 80 mg/g CY was injected intraperitoneally for 3 consecutive days.On the 4th day,mice in blank group and model group were intraperitoneally injected with 0.3 mL normal saline every day,mice in positive group were intraperitoneally injected with 200 mg/g APS suspension,mice in high-dose,medium-dose and low-dose SeGUP groups were intraperitoneally injected with 100,200 and 300 mg/g SeGUP suspension for 7 d.The results showed that the body weight of mice in high-dose SeGUP group and medium-dose SeGUP group was extremely higher than that in low-dose SeGUP group(P<0.01).The K andαin high-dose SeGUP group and medium-dose SeGUP group were extremely higher than those in low-dose SeGUP group(P<0.01).The serum IL-2 content of mice in high-dose SeGUP group was extremely higher than that in medium-dose SeGUP group and positive SeGUP group(P<0.01),and the serum IL-2 content of mice in medium-dose SeGUP group and positive SeGup group was extremely higher than that in low-dose Segup group and blank group(P<0.01).The content of TNF-α,IgM,IFN-γand IgG in serum in high-dose SeGUP groupand medium-dose SeGUP group were extremely higher than those in low-dose SeGUP group(P<0.01).The thymus index in high-dose SeGUP group and medium-dose SeGUP group was extremely higher than that in low-dose SeGUP group(P<0.01).The study indicates that SeGUP can enhance immune function of immunosuppressed mice by down-regulating spleen index,up-regulating body weight,K,α,IL-2,TNF-α,IgM,IFN-γ,IgG,and thymus index,and 200 and 300 mg/g of SeGUP is better.