抗大肠杆菌卵黄抗体及其纳米粒-微球对大肠杆菌腹泻预防效果评价

Evaluation on the effectiveness of anti Escherichia coli IgYand IgY nanoparticle-in-microsphere in the prevention of Escherichia coli-induced diarrhea

试验旨在探讨抗大肠杆菌卵黄抗体(IgY)及其纳米粒-微球对大肠杆菌腹泻的预防效果。采用IgY连续饲喂小鼠8 d,记录并计算小鼠体重变化、血常规变化、器官指数、巨噬细胞吞噬指数和吞噬百分数;建立小鼠大肠杆菌腹泻模型,将小鼠随机分为IgY组、纳米粒-微球组、空白对照组,分别以口服、腹腔注射、腹腔注射+口服3种方式给药,其中空白对照组以磷酸缓冲盐(PBS)代替IgY。结果显示,IgY饲喂试验中,IgY组小鼠的平均日增重高于对照组,增幅为11.25%;IgY组小鼠的肝脏指数、脾脏指数、巨噬细胞吞噬百分数显著高于对照组(P<0.05),IgY组小鼠的巨噬细胞吞噬指数极显著高于对照组(P<0.01)。在小鼠腹泻预防试验中,IgY与IgY纳米粒-微球对小鼠大肠杆菌腹泻均具有一定的保护作用,IgY组口服、腹腔注射、腹腔注射+口服3种给药方式下小鼠死亡率分别为16.67%、33.33%、0,IgY纳米粒-微球组对应给药方式的死亡率为83.33%、0、16.67%。研究表明,IgY对小鼠的生长及免疫具有促进作用,抗大肠杆菌IgY及其纳米粒-微球对小鼠大肠杆菌腹泻有显著的预防作用。

The experiment was to investigate the preventive effect of anti Escherichia coli IgY and IgY nanoparticle-inmicrosphere in the prevention of Escherichia coli-induced diarrhea.Mice were fed with IgY continuously for 8 d,and the trend of their weight,changes in blood routine,organ index,phagocytic index of macrophages and phagocytic percentage were recorded.Established Escherichia coli diarrhea model of mice.Mice were randomly divided into IgY group,nanoparticles microspheres group and control group,then given drugs in three ways of oral administration,intraperitoneal injection,intraperitoneal injection+oral administration.The control group used PBS to replace IgY.The results showed that in the IgY feeding experiment,the average daily weight gain of mice in IgY group was higher than that of control group,with an increase of 11.25%.The liver index,spleen index and macrophage phagocytosis percentage of the IgY group were significantly higher than those of control group(P<0.05),and the macrophage phagocytosis index of IgY group was extremely higher than that of control group(P<0.01).In the diarrhea prevention test,both IgY and IgY nanoparticle-in-microsphere had a certain protective effect.The mortality rates of mice in IgY group under oral administration,intraperitoneal injection,intraperitoneal injection+oral administration were 16.67%,33.00%and 0,the corresponding mortality rates for IgY nanospheremicrosphere group were 83.33%,0 and 16.67%.The study indicates that IgY and IgY-NiSMs have obvious prevention effects in diarrhea model of mice.