MC5R介导鹅肌肉对营养/能量水平变化的响应

MC5R mediates the response of goose muscle to changes in nutrition/energy levels

为探究黑皮质素受体MC5R在鹅营养/能量代谢中的作用及其机制,着重检测禁食/再饲喂模型中鹅胸肌和营养/能量代谢相关因子处理后鹅原代胸肌细胞中MC5R的mRNA表达水平,通过过表达MC5R后的转录组测序分析筛查MC5R调控的基因与通路,以及检测活体和细胞模型中MC5R下游基因的mRNA表达量。结果表明:肌肉中MC5R的表达为禁食所诱导,而这种诱导可被再饲喂所抑制;鹅原代肌细胞中MC5R的表达可被葡萄糖和油酸所诱导,但被甲状腺素所抑制;MC5R过表达影响的差异表达基因主要富集于糖脂代谢和炎症相关的通路;在鹅活体和细胞模型中筛选到的PLA2G4A、PTGS2、TRAF2、IL6和PLPP4基因可能介导MC5R的生物学作用。综上,MC5R可能通过糖脂代谢和炎症相关通路介导鹅肌肉中营养/能量水平变化所引起的生物学效应。

To explore the role and mechanism of MC5R in goose nutrition/energy metabolism,this study was focused on determining the mRNA expression level of MC5R in the pectoral muscle of goose in the fasting/refeeding model and that in goose primary pectoral myocytes treated with factors related to nutrition/energy metabolism.After the overexpression of MC5R gene,the genes and pathways regulated by MC5R were identified by transcriptome sequencing analysis.In addition,the mRNA expression of downstream genes of MC5R was also determined in vivo and cell models.The data showed that the expression of MC5R in muscle was induced by fasting,and this induction could be inhibited by refeeding;the expression of MC5R in goose primary myocytes was induced by glucose and oleic acid but inhibited by thyroxine;the differentially expressed genes affected by MC5R overexpression were mainly enriched in pathways related to glucolipid metabolism and inflammation;the genes including PLA2G4A,PTGS2,TRAF2,IL6 and PLPP4 were identified as those possibly mediating the biological function of MC5R in living geese and the cell model.In conclusion,MC5R may mediate the biological effects induced by changes of nutrition/energy levels in goose muscle through the pathways related to glucolipid metabolism and inflammation.