丙酮酸钠对小鼠海马神经HT22细胞神经保护作用

The neuroprotective effect of sodium pyruvate on mouse hippocampal neural HT22 cells

目的研究丙酮酸钠(sodium pyruvate,SP)在低氧条件下对小鼠海马神经细胞HT22的神经保护作用。方法在低氧条件下,用MTS检测不同浓度SP孵育HT22细胞的活性变化;从形态学上,采用铁染色观察SP对HT22细胞的影响;应用溶酶体染色检测SP对HT22细胞的溶酶体变化;采用Western blot检测Bcl-2、Bax和LC3-Ⅱ/LC3-Ⅰ蛋白的表达。结果5 mmol·L^(-1)的SP能提高HT22细胞活力以及减少HT22细胞损伤;SP提高Bcl-2的表达,降低Bax的表达,使LC3-Ⅱ/LC3-Ⅰ比值增加。结论在低氧的条件下给予SP可能通过减少HT22细胞凋亡,激活自噬发挥神经保护作用,从而降低低氧损伤。

Aim To study the effect of sodium pyruvate on apoptosis and autophagy of HT22 in mouse hippocampal neuronal cells under hypoxia conditions.Methods HT22 cells were incubated with different concentrations of sodium pyruvate to detect their cellular activity by MTS;iron staining was used to further observe the effect of sodium pyruvate on HT22 cells in mitochondrial metabolism;lysosomal staining was applied to detect the lysosomal changes of sodium pyruvate on HT22 cells;Western blot was used to detect the expression of Bcl-2,Bax and LC3-II/LC3-Ⅰ proteins.Results To verify whether sodium pyruvate exerted neuroprotective effects on mouse hippocampal HT22 cells through affecting mitochondrial apoptosis and autophagy pathways,which were improved by administration of sodium pyruvate.Conclusions Sodium pyruvate administration under hypoxic conditions can reduce the neuroprotective effect of hypoxic injury by reducing apoptosis and activating autophagy in HT22 cells.