摘要
目的:研究基于类酶活性二氧化锰纳米片(MnO_(2)NS)的新型传感应用于血清中胆固醇(Chol)的检测效果。方法:以四甲基氢氧化铵、过氧化氢和氯化锰为原料制备得到MnO_(2)NS。利用Chol和胆固醇氧化酶(ChOx)的氧化反应和MnO_(2)NS催化3,3’,5,5’-四甲基联苯胺(TMB)显色反应,根据反应后溶液颜色及吸光度的变化对Chol进行定量检测,并对反应温度、pH以及反应时间等条件进行了优化。结果:成功合成了类酶活性的MnO_(2)NS。该方法用于Chol的检测比传统MnO_(2)NS比色法具有更优的分析性能。在最优的实验条件下,Chol的检测线性范围为1~200μmol/L,检测限为0.5μmol/L,且具有较高的选择性。此外,该方法用于人血清中Chol的检测结果令人满意。结论:该传感器操作简单、快捷,具有较高的特异性与灵敏度,可为Chol相关的临床诊断提供新的检测方法。
Objective:To investigate the effect of a novel sensor based on manganese dioxide nanosheets(MnO_(2)NS)with enzyme-like activity for the detection of cholesterol(Chol)in human serum.Methods:MnO_(2)NS was prepared using tetramethylammonium hydroxide,hydrogen peroxide and manganese chloride as raw materials.The oxidation reaction of Chol and cholesterol oxidase(ChOx)and the colorimetric reaction of 3,3’,5,5’-tetramethylbenzidine(TMB)catalyzed by MnO_(2)NS were used to detect Chol quantitatively according to the changes in the color and the absorbance of the solution,and the reaction temperature,pH and reaction time were optimized.Results:MnO_(2)NS with enzyme-like activity was successfully synthesized.Compared to the traditional MnO_(2)NS colorimetric method,this method for Chol detection had better analytical performance.Under the best experimental conditions,the linear detection range was from 1μmol/L to 200μmol/L for Chol,with a detection limit of 0.5μmol/L and high selectivity.In addition,the method had achieved satisfactory results for Chol detection in human serum.Conclusion:The sensor is simple and easy to operate,with high specificity and sensitivity,providing a new detection method for Chol-related clinical diagnosis.
作者
赖荣基
汪贝贝
陈婷婷
覃英凤
刘金文
Lai Rongji;Wang Beibei;Chen Tingting;Qin Yingfeng;Liu Jinwen(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,The Key Laboratory of Biological Molecular Medicine Research in Guangxi Universities,Guangxi Medical University,Nanning 530021,China;Key Laboratory of Longevity and Aging-related Diseases Ministry of Education,Guangxi Medical University,Nanning 530021,China;Department of Human Anatomy,School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China)
出处
《广西医科大学学报》
CAS
2023年第6期1055-1061,共7页
Journal of Guangxi Medical University
基金
国家自然科学基金资助项目(No.22266007)
广西高校中青年教师科研基础能力提升项目(No.2022KY0098)。