LC-MS/MS法测定水性涂料中MI的基质干扰问题消除方法研究

Study on Elimination of Matrix Effect on Determination of MI in Waterborne Coatings by LC-MS/MS

通过固相萃取法、固相辅助液液萃取法、不同离子源的样品溶液稀释法、标准加入法等4种方法研究了水性涂料样品中MI测定时的基质干扰问题。固相萃取法和固相辅助液液萃取法的测定结果均较液相色谱法(LC法)大幅偏低,仍存在较大的基质干扰问题。标准加入法的测定结果与LC法基本一致,说明此法可解决基质干扰问题。不同离子源的样品溶液稀释法在一定条件下可将基质干扰降至可忽略的程度。ESI源在样品溶液稀释100倍时测定结果与LC法基本一致,APCI源在样品溶液稀释25倍时测定结果与LC法基本一致。标准加入法与不同离子源的样品溶液稀释法相比操作繁琐,后者更加适合大批量样品的同时处理。

The matrix effect on the determination of MI in waterborne coating samples was studied by four methods:solid phase extraction,solid-supported liquid/liquid extraction,sample solution dilution method with different ion sources,and standard addition method.The determination results of solid phase extraction and solid-supported liquid/liquid extraction are significantly lower than that of LC method,revealing that a noticeable matrix effect problem occurs.The determination results of standard addition method are basically consistent with that of LC method,indicating that this method can solve the matrix effect problem.The sample solution dilution method with different ion sources can reduce the matrix effect to a negligible degree under certain conditions.The determination results of ESI source are basically consistent with that of LC method when the sample solution is diluted by 100 times.The determination results of APCI source are basically consistent with that of LC method when the sample solution is diluted by 25 times.The standard addition method is more complicated than the sample solution dilution method with different ion sources,and the latter is more suitable for the simultaneous treatment of large batches of samples.