lncRNA C5orf66-AS1通过调节CYC1表达对胃癌细胞增殖和转移的影响

Effects of lncRNA C5orf66-AS1 on the proliferation and metastasis of gastric cancer cells by regulating the expression of CYC1

目的:探讨长链非编码RNA C5orf66反义链1(lncRNA C5orf66-AS1)对胃癌细胞增殖和转移的影响。方法:以2019年6月—2021年6月收集的63例胃癌患者的癌组织与癌旁组织、人胃黏膜细胞GES-1及胃癌细胞BGC823、SGC7901、AGS、MKN28为研究对象,分别检测组织和细胞中C5orf66-AS1和细胞色素c1(CYC1z)蛋白表达情况。将AGS细胞分为7组,分别检测各组细胞增殖、迁移、侵袭以及细胞中C5orf66-AS1表达水平和CYC1、细胞周期蛋白D1(CyclinD1)、基质金属蛋白酶-2(MMP-2)、MMP-9蛋白表达情况。结果:与癌旁组织比较,胃癌组织中C5orf66-AS1表达降低,CYC1蛋白表达升高(P<0.05)。与人胃黏膜细胞GES-1比较,胃癌细胞BGC823、SGC7901、AGS、MKN28中C5orf66-AS1表达降低,CYC1蛋白表达升高(P<0.05),且AGS细胞中C5orf66-AS1表达最低,CYC1蛋白表达水平最高。沉默C5orf66-AS1促进AGS细胞增殖、迁移、侵袭及CYC1、CyclinD1、MMP-2、MMP-9蛋白表达;过表达C5orf66-AS1抑制AGS细胞增殖、迁移、侵袭及CYC1、CyclinD1、MMP-2、MMP-9蛋白表达;过表达CYC1可逆转C5orf66-AS1对AGS细胞增殖、迁移、侵袭的抑制作用。结论:过表达C5orf66-AS1可能通过下调CYC1抑制AGS细胞增殖、迁移和侵袭。

Objective:To investigate the effects of long non-coding RNA C5orf66 antisense strand 1(lncRNA C5orf66-AS1)on the proliferation and metastasis of gastric cancer cells.Meth ods:The cancer tissues and paracancerous tissues from 63 patients with gastric cancer collected in this hospital from June 2019 to June 2021,human gastric mucosal cells GES-1 and gastric cancer cells BGC823,SGC7901,AGS,and MKN28 were used as the research objects,the expressions of C5orf66-AS1 and cytochrome c1(Cyc1)proteins in tissues and cells were detected.The AGS cells were divided into seven groups,the cell proliferation,migration,invasion and the expression of C5orf66-AS1,CYC1,CyclinD1,MMP-2,MMP-9 were detected in each group.Results:Compared with adjacent tissues,the expression of C5orf66-AS1 in gastric cancer tissue was reduced,and the expression of CYC1 protein was increased(P<0.05).Compared with human gastric mucosal cells GES-1,the expression of C5orf66-AS1 in gastric cancer cells BGC823,SGC7901,AGS,and MKN28 was reduced,and the expression of CYC1 protein was increased(P<0.05),the AGS cells had the lowest expression of C5orf66-AS1 and the highest expression level of CYC1 protein,therefore.silencing C5orf66-AS1 promoted AGS cell proliferation,migration,invasion and CYC1,CyclinD1,MMP-2,MMP-9 protein expression;overexpression of C5orf66-AS1 inhibited AGS cell proliferation,migration,invasion and CYC1,CyclinD1,MMP-2,MMP-9 protein expression;overexpression of CYC1 could reverse the inhibitory effects of C5orf66-AS1 on the proliferation,migration and invasion of AGS cells.Conclusion:Overexpression of C5orf66-AS1 may inhibit the proliferation,migration and invasion of AGS cells by down-regulating CYC1.