外源茉莉酸甲酯对温室牡丹‘洛阳红’挥发性成分及含量的影响

Effects of Exogenous Methyl Jasmonate on Volatile Components and Content of Paeonia suffruticosa‘Luoyanghong’in Greenhouse

【目的】探讨外源茉莉酸甲酯对温室牡丹‘洛阳红’挥发性成分及含量的影响,获得改善温室牡丹花朵香气成分的方法,为提高牡丹的经济效益提供参考。【方法】采用整株喷施法,以5种不同浓度(50、100、200、300、400μmol·L^(-1))的茉莉酸甲酯溶液喷施温室牡丹‘洛阳红’植株,利用动态顶空套袋吸附法结合气相色谱—质谱联用技术检测其不同开花时期的挥发性成分及含量。【结果】1)不同开花时期的温室牡丹‘洛阳红’共检测出25种挥发性化合物,其中未喷施和喷施茉莉酸甲酯溶液的植株分别检测到23种和24种化合物。可将这些挥发性化合物分为醇类、酯类、醛类、萜烯类、苯环类和烃类6大类,其中烃类和苯环类化合物的总释放量相对较高;喷施不同浓度的茉莉酸甲酯溶液后,六类化合物含量在不同开花时期呈现不同变化趋势。2)对香气化合物的分析发现,对二甲氧基苯、2,4-二叔丁基苯酚、茉莉酸甲酯这3种化合物在大多数样品中含量较高,可视为温室牡丹‘洛阳红’的主要香气成分。3)喷施茉莉酸甲酯溶液后,对二甲氧基苯含量下降,2,4-二叔丁基苯酚和茉莉酸甲酯含量升高,浓度以100μmol·L^(-1)为最佳。【结论】喷施浓度为100μmol·L^(-1)的茉莉酸甲酯溶液能较好地改变温室牡丹‘洛阳红’香气成分及含量,从而改善‘洛阳红’置于室内时因香气过于浓郁而令人感到不适的问题。

【Objective】Paeonia suffruticosa‘Luoyanghong’is one of common varieties with flower forcing,however the flower fragrance is too strong,and can make people feel uncomfortable when it is placed indoors.The aim of this study is to explore effects of exogenous methyl jasmonate on the volatile components and content and to obtain a method to improve the aroma components of P.suffruticosa‘Luoyanghong’in greenhouse,so as to provide a reference for improving the economic benefits of peony.【Method】The whole plant of P.suffruticosa‘Luoyanghong’grown in greenhouse was sprayed with methyl jasmonate solutions(50,100,200,300,400μmol∙L^(−1)).The dynamic headspace adsorption method in combination with gas chromatography-mass spectrometry technology was adopted to detect the volatile components and content of flowers at different flowering stages.【Result】1)A total of 26 volatile compounds were detected in P.suffruticosa‘Luoyanghong’in greenhouse at different flowering stages.There were 23 compounds in the plants without methyl jasmonate treatment and 25 compounds in the plants sprayed with methyl jasmonate solution.These volatile compounds could be divided into six categories:alcohols,esters,aldehydes,terpenes,benzene rings and hydrocarbons,among them the total release amount of hydrocarbons and benzene rings was relatively high.After being sprayed with methyl jasmonate,the content of the six compounds showed different trends at different flowering stages.2)The analysis of aroma compounds found that the three compounds,1,4-dimethoxybenzene,2,4-di-tertbutylphenol and methyl jasmonate,showed relatively high levels in most samples,which could be regarded as the main aroma component of P.suffruticosa‘Luoyanghong’in greenhouse.3)After being sprayed with methyl jasmonate solution,the content of 1,4-dimethoxybenzene decreased,while the content of 2,4-di-tert-butylphenol and methyl jasmonate increased.The best concentration for methyl jasmonate solution was 100μmol∙L^(−1).【Conclusion】Application of 100μmol∙L^(−1)methyl jasmonate solution can change the aroma components and content of the P.suffruticosa‘Luoyanghong’in greenhouse,so as to solve the uncomfortable feel problem of P.suffruticosa‘Luoyanghong’because of its strong fragrance when it is placed indoors.