海藻多肽的提取分离及抗肿瘤活性研究进展

Progress on extraction,isolation and antitumor activity of seaweed polypeptides

海藻是重要的海洋生物,也是人们食物的重要来源.前期广泛的研究已经证实,海藻含有大量的蛋白质.但由于多肽含量低,在提取纯化时,传统的方法存在一定难度,目前较常用酶解法和化学提取法进行提取,用色谱法和膜分离法进行纯化,从海藻中获取较高纯度的多肽,使用与MS相关的技术对其结构进行鉴定.海藻多肽对抗肿瘤具有较强作用,其作用机制表现在多个方面.本文综述了海藻多肽最新的提取纯化和鉴定方法及抗肿瘤活性的研究进展,为其进一步开发利用提供参考.

Seaweed is an important marine life and an important source of food for people.Extensive previous studies have confirmed that seaweed contains a lot of protein.However,due to the low content of polypeptides,polypeptides are difficult to be extracted and purified by the traditional methods.At present,enzymatic hydrolysis and chemical extraction are more commonly used for extraction,and chromatography and membrane separation are used for purification.A higher purity polypeptides could be obtained from seaweed,and its structure was identified using MS-related technique.Seaweed polypeptides have a strong effect against tumor,and the mechanisms are manifested in many aspects.In this paper,the latest extraction,purification and identification methods of seaweed polypeptides,as well as the latest research progress of their anti-tumor activity were reviewed,so as to provide reference for their further development and utilization.