藿香中的两个新乌苏烷型三萜

Two new ursane triterpenoids from Agastache rugosa (Fisch. et.Mey.) O. Kuntze

采用硅胶、MCI、ODS柱色谱和高效制备液相色谱等方法,从藿香80%乙醇提取物中分离得到2个新乌苏烷型三萜和12个已知化合物。通过质谱、核磁共振和ECD等波谱学方法鉴定新化合物的结构分别为2α,3α-dihydroxy-24-nor-urs-4(23),12(13)-dien-28-oic acid (1)和2α,3α-dihydroxy-24-nor-urs-4(23),12(13), 20(30)-trien-28-oic acid (2),命名为agasursacid A和agasursacid B。对分离得到的部分化合物测定了抗柯萨奇病毒B3型(CVB3)活性,化合物3、4、6、8对柯萨奇病毒B3型有抑制活性, IC50值分别为4.77、1.59、11.11、25.87μmol·L^(-1)。

Two new ursane triterpenoids along with twelve known compounds were isolated from 80%ethanol extract of Agastache rugosa(Fisch.et.Mey.)O.Kuntze by using silica gel column,MCI column,ODS column and HPLC.The structures of the new compounds were identified as 2α,3α-dihydroxy-24-nor-urs-4(23),12(13)-dien-28-oic acid(1)and 2α,3α-dihydroxy-24-nor-urs-4(23),12(13),20(30)-trien-28-oic acid(2)by HR-ESI-MS,NMR and ECD spectral data,named agasursacid A and agasursacid B.In addition,compounds 3,4,6,8 showed anti-coxsackievirus B3(CVB3)activities with a IC50 as 4.77,1.59,11.11 and 25.87μmol·L^(-1),resepectively.