基于植物代谢组学和靶标定量分析研究山西不同产地黄芩叶化学成分差异

Study on the differences in chemical composition of Scutellaria baicalensis leaves from different origins in Shanxi Province based on plant metabolomics and targeted quantitative analysis

本研究整合植物代谢组学和靶标定量分析方法,系统分析山西不同产地间黄芩叶化学成分的差异,为合理有效利用黄芩叶资源提供理论依据。基于植物代谢组学的思路,采用UPLC-QTOF-MS对山西省8个不同产地共53批黄芩叶进行液质分析,并通过多元统计分析方法,筛选山西不同产地间的差异化学成分。同时,优化并建立一种可以同时测定黄芩叶中7种黄酮类成分和3种有机酸类成分的含量测定方法,以定量分析山西不同产地黄芩叶化学成分差异。植物代谢组学结果显示,晋北(大同、忻州)、晋中(阳泉、吕梁)和晋南(长治、运城、晋城、临汾)三个区域的黄芩叶化学成分组成具有差异性:晋北和晋中地区间存在14个显著差异化学成分;晋南和晋中之间存在18个显著差异化学成分;晋北和晋南之间存在15个显著差异化学成分。其中,野黄芩苷和异红花素-7-O-葡萄糖醛酸苷是三个地区间的共有差异成分,野黄芩苷在晋南地区含量最高,在晋北地区含量最低;异红花素-7-O-葡萄糖醛酸苷在晋中地区含量最高,在晋北地区含量最低。定量分析结果进一步证实,晋北地区芹菜素、柚皮素和柠檬酸的平均含量最高,晋南地区野黄芩苷、咖啡酸、芹菜素-7-O-葡萄糖醛酸苷、苹果酸和汉黄芩苷的平均含量最高,晋中地区汉黄芩素和黄芩苷的平均含量最高。该研究对于黄芩叶资源的质量控制具有重要意义,为合理有效利用黄芩叶资源提供理论依据。

By integrating plant metabonomics and target quantitative analysis methods,this study systematically analyzed the differences of chemical constituents in Scutellaria baicalensis leaves from different producing areas in Shanxi,so as to provide theoretical basis for rational and effective utilization of Scutellaria baicalensis leaves.Based on the idea of plant metabonomics,the liquid quality of 53 batches of Scutellaria baicalensis leaves from 8 different producing areas in Shanxi was analyzed by UPLC-QTOF-MS,and the collected data were imported into SIMCA 14.1 software for multivariate statistical analysis to screen the different chemical constituents among different habitats in Shanxi.Meanwhile,a method for simultaneous determination of 7 flavonoids and 3 organic acids in Scutellaria baicalensis leaves was optimized and established to quantitatively analyze the differences of chemical components in Scutellaria baicalensis leaves from different producing areas in Shanxi.The results of plant metabonomics showed that there were differences in the chemical composition of Scutellaria baicalensis leaves in northern Shanxi(Datong,Xinzhou),Jinzhong(Yangquan,Luliang)and southern Shanxi(Changzhi,Yuncheng,Jincheng,Linfen):there were 14 significant differences in chemical composition between northern Shanxi and Jinzhong;there were 18 significant differences in chemical constituents between southern Shanxi and central Shanxi.There were 15 significant differences in chemical constituents between northern Shanxi and southern Shanxi.Among them,scutellarin and isocarthamidin-7-O-glucuronide were the common differences among the three regions,and the content of scutellarin was the highest in southern Shanxi and the lowest in northern Shanxi.The content of isocarthamidin-7-O-glucuronide was the highest in Jinzhong area and the lowest in northern Shanxi area.Quantitative analysis further confirmed that the average contents of apigenin,naringenin and citric acid were the highest in northern Shanxi,scutellarin,caffeic acid,apigenin-7-O-glucuronide,malic acid and wogonoside were the highest in southern Shanxi,and wogonoside and baicalin were the highest in central Shanxi.This study is of great significance to the quality control of Scutellaria baicalensis leaf resources,and provides theoretical basis for rational and effective utilization of Scutellaria baicalensis leaf resources.