牛超数排卵-体内胚胎生产影响因素的研究

Study on Influencing Factors of Superovulation-Embryo Production in Bovine

该试验旨在分析牛超数排卵-体内胚胎生产效果的影响因素,为提牛体内胚胎生产效率、优化体内胚胎生产流程提供生产依据。14~15月龄繁殖性能正常的荷斯坦牛和西门塔尔牛青年母牛进行了超数排卵处理,并就不同品种,不同种类促卵泡激素,重复超排牛只和不同操作人员的超排效果进行了统计比较。西门塔尔牛的平均回收胚胎数和平均有效胚胎数高于荷斯坦牛,分别为差异显著(P<0.05)和差异不显著(P>0.05)。荷斯坦的有效胚胎率高于西门塔尔牛,但差异不显著(P>0.05);进口促卵泡激素处理后的西门塔尔牛获得的平均胚胎和平均有效胚胎及有效胚胎率均显著高于国产促卵泡激素注射组(P<0.05)。从不同国家进口的促卵泡激素组之间差异不显著(P>0.05);不同的技术人员操作获得的平均胚胎数之间差异不显著(P>0.05),有效胚胎数和有效胚胎率1号与2号技术员之间无显著差异(P>0.05),但是显著高于3号技术员的操作结果(P<0.05);同一批牛前后两次超排获得的平均胚胎数分别为7.0枚和7.7枚,平均有效胚胎分别为2.7枚和3.2枚,有效胚胎率分别为38%和41%,第二次超排效果虽优于第一次但是两次超排效果差异不显著(P>0.05)。超数排卵和体内胚胎生产受供体牛品种、超排次数、促卵泡激素来源、操作人人员技术水平等多方面因素影响,在良好的生产管理水平下,综合各种因素在内找到最优的超排方案。

The purpose of the experiment was to analyze the influencing factors of bovine superovulation-in vivo embryo production,and to provide basis for improving in vivo embryo production efficiency and optimizing in vivo embryo production process.Holstein and Simmental cattle young cows with normal reproductive performance at 14~15 months of age were subjected to superovulation treatment,and different breeds and types of Follitropin beta,the superovulation effect of repeated supersteak was compared with that of different operators.The mean number of recovered embryos and the mean number of effective embryos in the Simmental cattle were higher than those in the Holstein,with significant difference(<0.05)and no significant difference(>0.05),respectively.The effective embryo rate of Holstein was higher than that of Simmental cattle,but the difference was not significant(>0.05)The average embryo,effective embryo and effective embryo rate of the imported Follitropin beta treated Simmental cattle were significantly higher than those of the domestic Follitropin injection group(<0.05).There was no significant difference in the average number of embryos obtained from Follitropin beta imported from different countries(>0.05),and no significant difference in the average number of embryos obtained by technicians from different countries(>0.05),there was no significant difference in the number of effective embryos and effective embryo rate between technician No.1 and technician No.2(>0.05),but it was significantly higher than that of technician No.3(<0.05)The average number of embryos obtained by superovulation was 7.0 and 7.7,the average number of effective embryos was 2.7 and 3.2,the effective embryo rate was 38%and 41%,respectively,the effect of the second superovulation was better than that of the first,but there was no significant difference between the two superovulation(P>0.05).Superovulation and in vivo embryo production are affected by various factors such as donor breed,superovulation frequency,source of Follitropin beta,technical level of operators,etc.,the optimal superovulation scheme is found by integrating various factors.