一例荷斯坦犊牛支原体性肺炎和关节炎的诊断

Diagnosis of Mycoplasma Pneumonia and Arthritis in a Holstein Calf

本研究通过临床病史、尸体剖检、组织病理学、PCR和基因扩增及测序方法诊断了1例荷斯坦犊牛支原体性肺炎和关节炎。发病犊牛消瘦,腕关节、膝关节和跗关节明显肿大,呼吸困难,病程15 d。尸体剖检见浆液纤维素性关节炎和关节周围炎,局部脓肿;支气管性大叶性间质性肺炎;肾脏表面散在小出血点。组织病理学见慢性增生性间质性肺炎、浆液纤维素性化脓性关节炎和关节周围炎。取犊牛关节液培养72 h,见典型“油煎蛋”样支原体菌落。提取肺组织和关节液基因组,用支原体通用引物扩增出1500 bp左右的目的条带,测序后在GenBank数据库中比对,均与湖北牛支原体参考株同源性最高,同源率在99%以上。

In this study,a case of mycoplasma pneumonia and arthritis in Holstein calves was diagnosed by clinical history,necropsy,histopathology,PCR,gene amplification and sequencing methods.The affected calves were emaciated,with significantly enlarged wrist,knee and tarsus,and respiratory difficulties with a duration of 15 d.At necropsy,serous cellulose arthritis and periarticular tis,local abscess,bronchial lobar interstitial pneumonia,small bleeding spots on the renal surface.Histopathology was seen in chronic hyperplastic interstitial pneumonia,serous cellulose purulent arthritis and periarticular tis.Calf joint solution was collected for 72 h in typical"fried egg"-like mycoplasma colonies.The genome of lung tissue and joint fluid was extracted,and the target band of about 1500 bp was amplified with mycoplasma universal primers.After sequencing it was compared in the GenBank database,which had the highest homology with Hubei Mycoplasmabovis reference strain,and the homology rate was above 99%.