牛病毒性腹泻的流行病学调查与防控

Epidemiological Investigation and Control of Bovine Viral Diarrhea in Dairy Farm

[目的]了解陕西省榆林市奶牛场牛病毒性腹泻病毒(BVDV)感染情况。[方法]从5家奶牛场采集156份血清样品,采用双抗体夹心ELISA方法进行BVDV抗原检测。[结果]除1家未检测到BVDV,其余4家均存在BVDV感染,BVDV抗原阳性率0~6.38%,平均为4.49%(7/156),成母牛、犊牛、育成牛BVDV抗原阳性率分别为5.66%、4.44%、3.45%。[结论]陕西省榆林市5家奶牛场存在一定的BVDV流行和持续性感染牛,且感染率较高,建议奶牛场采取定期检测、引种检疫、免疫接种等综合防控方法,以有效净化BVDV。

[Objective]To understand the prevalence of Bovine viral diarrhea virus(BVDV)infection at Yulin dairy farms in Shaanxi Province.[Method]156 serum samples were collected from 5 dairy farms and BVDV antigen was detected by double antibody sandwich ELISA.[Result]BVDV infection was found in the four families.The positive rates of BVDV antigen ranged from 0 to 6.38%,with an average of 4.49%(7/156).The positive rates of BVDV antigen in adult cows,calves and raise cows were 5.66%,4.44%and 3.45%respectively.[Conclusion]BVDV was prevalent and persistently infected in cattle from 5 dairy farms in Yulin,Shaanxi Province.The infection rate of BVDV was high.Therefore,effective purification measures of BVDV such as periodic detection,introduction and quarantine,immunization were put forward.