奶牛乳房炎病原菌分离鉴定及药敏试验——以甘肃天水为例

Isolation and Identification of Bacterial Pathogens and Drug Sensitivity Test of Dairy Cow Mastitis——Take Tianshui of Gansu as an example

[目的]为了解甘肃天水奶牛乳房炎病原菌种类及对药物的敏感性。[方法]选取甘肃天水3个奶牛场40头患有临床乳房炎奶牛乳样,分别采用培养基法和生化分析法进行病原菌分离和鉴定;采用Kirby-Bauer纸片扩散法对分离得到的病原菌进行体外药物敏感性试验。[结果]在分离的140株病菌中,大肠杆菌、金黄色葡萄球菌和无乳链球菌数量最多,分别占32.1%、27.2%和14.3%。药敏试验结果显示,分离得到的45株大肠杆菌对青霉素均呈现耐药,耐药率高达100%,而大肠杆菌对万古霉素的敏感性最高,45株菌株中未检测到耐药性。[结论]为甘肃天水奶牛乳房炎的防治提供一定的理论依据。

[Objective]To understand the types of pathogens causing mastitis in dairy cows in Tianshui,Gansu and their sensitivity to drugs.[Method]40 milk samples from clinical mastitis cows from 3 dairy farms in Tianshui,Gansu were selected,and pathogenic bacteria were isolated and identified using culture medium method and biochemical analysis method,respectively.Kirby Bauer disk diffusion method was used to conduct in vitro drug sensitivity test on the isolated pathogenic bacteria.[Result]Among 140 strains of bacteria isolated,Escherichia coli,Staphylococcus aureus and Streptococcus agalactis accounted for 32.1%,27.2%and 14.3%respectively.The results of drug sensitivity test showed that 45 strains of Escherichia coli were resistant to penicillin,and the drug resistance rate was as high as 100%,while Escherichia coli was the most sensitive to Vancomycin,and no drug resistance was detected in 45 strains.[Conclusion]Provide a certain theoretical basis for the prevention and treatment of mastitis in Gansu Tianshui dairy cows.