钛种植体表面构建成骨细胞特异性识别多肽缓释系统的实验研究

The experimental study on the construction of osteoblast specific recognition polypeptide sustained-release system on titanium implant surface

目的利用LBL技术在碱热处理后的钛种植体表面构建海藻酸钠-壳聚糖聚电解质多层膜结构,并以此结构复合成骨细胞特异性识别多肽,探究其缓释效应。方法光滑钛表面经氢氧化钠碱热处理后通过层层自组装技术将海藻酸钠、壳聚糖进行交替吸附沉积,形成聚电解质多层膜结构,利用此多层膜装载成骨细胞特异性识别多肽,构建其缓释系统,并评价缓释效应。结果通过接触角测量仪、场发射扫描电子显微镜、荧光显微镜、X射线光电子能谱仪、傅里叶红外光谱以及紫外分光光度计对样品表面进行检测,证实在钛片表面成功构建海藻酸钠—壳聚糖多层膜结构,且成功装载成骨细胞特异性识别多肽,缓释实验显示,该多层膜结构缓释效果明显优于对照组,表明该多层膜结构对特异性多肽具备显著的缓释效应。结论钛种植体表面通过层层自组装形成多层膜结构并成功装载成骨细胞特异性多肽,构建该多肽的缓释系统,缓释效果显著,有望能够促进钛种植体表面骨结合。

Objective To construct the sodium alginate-chitosan polyelectrolyte multilayer membrane structure on the surface of alkaline heat treated titanium by LBL technology,and to combine the peptide that specifically bind to osteoblasts with this structure to explore their slow-release effects.Methods After the smooth titanium surface was heat treated with NaOH,sodium alginate and chitosan were alternately adsorbed and deposited on the surface by layer by layer self-assembly method to form a polyelectrolyte multilayer film structure,and the multilayer film was used to load the peptide that specifically bind to osteoblasts,construct slow-release system,and evaluate the slowrelease effect.Results The sample surface was tested by contact angle measuring instrument,field emission scanning electron microscope,fluorescence microscope,X-ray photoelectron spectrometer,Fourier transform infrared spectroscopy and ultraviolet spectrophotometer to confirm that the sodium alginate-chitosan multilayer film structure was successfully constructed on the surface of titanium sheets,and the peptide that specifically bind to osteoblasts were successfully loaded.Conclusion The surface of titanium implants is self-assembled layer by layer to form a multilayer membrane structure and successfully loaded with the peptide that specifically bind to osteoblasts,and the sustained release system of the peptide is constructed,which has a remarkable sustained release effect and is expected to promote osseointegration on the surface of titanium implants.