载硅烷和BMP-2的多孔钛表面构建及其成骨活性评价

Construction of porous titanium surface loaded with Silane and BMP-2 and evaluation of its osteogenic activity

目的探究钛材纳米多孔表面载硅烷和骨形态发生蛋白-2(BMP-2)的制备方法和制备表面促成骨能力评价。方法采用碱热处理、硅烷化修饰和BMP-2固定,构建硅烷和BMP-2修饰的纳米多孔表面,扫描电子显微镜、X射线光电子能谱、红外光谱和水接触角检测分别表征制备过程中表面形貌、元素、官能团和亲水性的变化,免疫荧光观察固定的BMP-2,细胞活性测试和细胞荧光染色分析表面促成骨细胞黏附和增殖能力,碱性磷酸酶活性评估促细胞成骨能力。结果材料表征手段证实硅烷和BMP-2成功固定在纳米多孔钛表面,细胞评价证实制备表面不但显著增强成骨细胞的黏附和增殖,而且大幅提升成骨细胞的成骨活性。结论该修饰方法构建的表面对成骨细胞具有更好的相容性,有望改善钛基种植体的骨整合能力。

Objective To study the preparation method of silane and bone morphogenetic protein-2(BMP-2)on the nano-porous titanium surface and evaluate the osteogenic ability of the prepared surface.Methods Silane and BMP-2 modified nano-porous titanium surfaces were constructed by alkali-heat treatment,silanization modification and BMP-2 grafting.Scanning electron microscopy,X-ray photoelectron spectroscopy,infrared spectroscopy and water contact angle tests were used to characterize the changes of surface morphology,elements,functional groups and hydrophilicity during the preparation process.Immunofluorescence was used to observe the BMP-2 grafted on the surface.Cell activity test and cell fluorescence staining were used to analyze the ability to promote osteoblast adhesion and proliferation,and alkaline phosphatase activity was used to evaluate the ability to promote cell osteogenesis.Results Material characterization results confirmed that silane and BMP-2 were successfully immobilized on the nano-porous titanium surface.Cell evaluation confirmed that the prepared surface not only significantly enhanced the adhesion and proliferation of osteoblasts,but also greatly enhanced the osteogenic ability of osteoblasts.Conclusion The constructed surface has better compatibility with osteoblasts and is expected to improve the osseointegration ability of titanium-based implants.