云南重楼果皮高效液相色谱指纹图谱研究

HPLC Fingerprint of Paridis Pericarpium

目的建立云南重楼果皮的高效液相色谱(HPLC)指纹图谱。方法色谱柱为Capcell PAK C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(梯度洗脱),流速为1.0 mL/min,检测波长为203 nm,柱温为30℃,进样量为15μL。以重楼皂苷Ⅶ峰为参照峰,绘制9批药材样品的HPLC指纹图谱,采用中药色谱指纹图谱相似度评价系统2.0版进行相似度评价,确定共有峰。结果建立了9批药材样品的HPLC指纹图谱,确定了16个共有峰,指认4个黄酮类成分及5个重楼皂苷类成分,相似度不小于0.903。结论所建立的方法专属性强、稳定性和重复性好,可用于云南重楼果皮的质量控制。

Objective To establish a high-performance liquid chromatography(HPLC)fingerprint of Paridis Pericarpium.Methods The chromatographic column was Capcell PAK C_(18)column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-aqueous(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was 203 nm,the column temperature was 30℃,and the injection volume was 15μL.With the peak of polyphyllinⅦas the reference peak,HPLC fingerprints of nine batches of samples were drawn,and the Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2.0)was used for similarity evaluation to determine the common peak.Results HPLC fingerprints of nine batches of samples were established with a similarity of≥0.903,16 common peaks were determined,and four flavonoids and five polyphyllins were identified.Conclusion The established method has strong specificity,stability,and good repeatability,which can be used for the quality control of Paridis Pericarpium.