经肝动脉灌注栓塞微球对比碘化油乳剂治疗VX2兔肝癌模型对VEGF、CTGF和HIFα-1的影响及疗效评估

Effects of DC Bead Drug Embolic Microspheres and Iodinated Oil Emulsion on VEGF,CTGF and HIFα-1 in VX2 Rabbit Liver Cancer Model and Efficacy Evaluation

目的:观察栓塞微球对比碘化油乳剂经肝动脉灌注化疗栓塞对VX2兔肝癌模型血管内皮生长因子(VEGF)、结缔组织生长因子(CTGF)和缺氧诱导因子(HIF)α-1的影响及疗效。方法:选取VX2兔肝癌模型24只,使用随机数字表法分为空白对照组、栓塞微球组、碘化油乳剂组,每组8只。经肝动脉分别灌注0.9%氯化钠溶液1 mL、栓塞微球0.5 mL+表柔比星溶液0.5 mL、碘化油乳剂0.5 mL+奥沙利铂0.5 mL,观察并比较各组VX2兔肝癌模型VEGF、CTGF和HIFα-1水平及病灶坏死情况。结果:行化疗药灌注及栓塞后,各组的VEGF、CTGF和HIFα-1水平均有不同程度升高。术后7、14 d,栓塞微球组、碘化油乳剂组的VEGF、CTGF和HIFα-1水平明显高于空白对照组,差异均有统计学意义(P<0.05)。术后28 d,栓塞微球组、碘化油乳剂组的Ⅱ—Ⅳ级病灶坏死率高于空白对照组。栓塞微球组与碘化油乳剂组病灶坏死率的差异无统计学意义(P>0.05)。结论:经肝动脉栓塞治疗后,因使用栓塞药物不同造成不同栓塞水平可能影响VEGF、CTGF和HIFα-1表达及病灶坏死率。

OBJECTIVE:To observe the effects of DC bead drug embolic microspheres and iodinated oil emulsion on vascular endothelial growth factor(VEGF),CTGF and hypoxia-inducing factor(HIF)α-1 in VX2 rabbit liver cancer model and efficacy evaluation.METHODS:Totally 24 VX2 rabbit liver cancer models were selected.The experimental animals were randomly divided into the blank control group,DC bead drug embolic microspheres group and iodinated oil emulsion group,with 8 cases in each group.The hepatic artery was perfused with 1 mL of 0.9%sodium chloride solution,0.5 mL of embolic microspheres+0.5 mL of epirubicin solution,0.5 mL of iodinated oil emulsion+0.5 mL of oxaliplatin.The levels of VEGF,CTGF,HIFα-1 and lesion necrosis rate of VX2 rabbit liver cancer model were observed and compared.RESULTS:The levels of VEGF,CTGF and HIFα-1 increased significantly after chemotherapy infusion and embolization.At 7 days and 14 days after surgery,the levels of VEGF,CTGF and HIFα-1 in DC bead drug embolic microspheres group and iodinated oil emulsion group were significantly higher than those in the blank control group,with statistically significant differences(P<0.05).At 28 days after surgery,the lesion necrosis rate in DC bead drug embolic microspheres group and iodinated oil emulsion group was higher than that in the blank control group.Yet there was no significant difference between DC bead drug embolic microspheres group and iodinated oil emulsion group(P>0.05).CONCLUSIONS:After treatment via hepatic artery embolization,different embolic levels caused by different embolic drugs may affect the expression difference of VEGF,CTGF and HIFα-1 and lesion necrosis rate.