番石榴遗传多样性的ISSR分析及指纹图谱构建

Psidium guajava L.:Genetic Diversity Analysis Based on ISSR Markers and Construction of Fingerprints

为研究59份番石榴种质资源的遗传多样性,并绘制指纹图谱。以番石榴为试材,利用ISSR分子标记技术和UPGMA聚类分析方法,绘制番石榴DNA指纹图谱。利用筛选获得的10条ISSR核心引物对59份番石榴样品进行扩增,共得到126条条带,其中多态性条带113条,多态率为89.68%。通过UPGMA聚类分析得知,59份供试番石榴种质资源样品间遗传相似系数为0.50~0.96,平均遗传相似系数为0.73。根据遗传距离的远近,可将59份种质资源分成4组,第1组包含种质34份,第2组包含种质6份,第3组包含种质18份,编号C26‘草莓’番石榴样品单独为第4组。成功构建了59份番石榴种质的DNA指纹图谱,可用于番石榴种质资源的分类与鉴定。

In order to study the genetic diversity of 59 guava germplasm resources and to map the fingerprint,DNA fingerprinting of guava was studied by ISSR technique and UPGMA cluster analysis.The 10 ISSR core primers obtained by screening were used to amplify 59 guavas samples,and a total of 126 bands were obtained,including 113 polymorphic bands,with a polymorphism rate of 89.68%.According to UPGMA clustering analysis,the genetic similarity coefficient of 59 guava germplasm resource samples ranged from 0.50 to 0.96,and the average genetic similarity coefficient was 0.73.According to the genetic distance,the 59 germplasm resources could be divided into four groups,group 1 contained 34 germplasm resources,group 2 contained 6 germplasm resources,group 3 contained 18 germplasm resources,and the sample number C26'strawberry'guava was the fourth group alone.The DNA fingerprints of 59 guava germplasm resources were successfully constructed,which could be used for the classification and identification of guava germplasm resources.