黄芪内生真菌HHQ14的初步鉴定及挥发性成分分析

Endophytic Fungus HHQ14 from Astragalus membranaceus:Preliminary Identification and Volatile Components Analysis

旨在高效利用黄芪内生真菌HHQ14,丰富其次生代谢产物资源。以黄芪内生真菌HHQ14为研究对象,采用菌株形态学观察以及ITS序列分析对其进行菌种鉴定,使用GC-MS法分析其挥发性成分。经鉴定,黄芪内生真菌HHQ14为木生锥毛壳菌(Coniochaeta ligniaria);经GC-MS法分析,从黄芪内生真菌HHQ14发酵液鉴定出6种挥发性成分,菌丝体中鉴定出13种挥发性成分,黄芪茎对照药材中鉴定出11种挥发性成分,黄芪根对照药材中鉴定了12种挥发性成分。其中黄芪内生真菌HHQ14发酵液与菌丝体中均含有与黄芪根、茎相同的挥发性成分2-((1R,6R)-3-甲基-6-(丙-1-烯-2-基)环己-2-烯基)-5-戊基苯-1,3-二酚,其菌丝体中还含有与黄芪根、茎相同的挥发性成分芥酰胺。内生真菌HHQ14为宿主植物黄芪次生代谢产物产生菌,并含有多种具有广泛用途及医疗价值的挥发性成分,上述结果为综合开发应用其次生代谢产物奠定基础。

In order to take advantage of utilizing the endophytic fungus HHQ14 which was isolated from Astragalus membranaceus,and to rich the resources of its secondary metabolites,the endophytic fungus HHQ14 was chosen as the research object,and was identified by morphological observation and ITS sequence analysis.Meanwhile,the volatile components were analyzed by GC-MS.The endophytic fungi HHQ14 were identified as Coniochaeta ligniaria and there were 6 volatile components in the fermentation broth of endophytic fungus HHQ14,13 volatile components were identified in the mycelium,11 volatile components were identified in the stem of Astragalus membranaceus and 12 volatile components were identified in the root of Astragalus membranaceus.Among them,the fermentation broth and mycelium of HHQ14 endophytic fungus contained the same volatile component 2-((1R,6R)-3-methyl-6-(propyl-1-alken-2-yl)cyclohexe-2-alkenyl)-5-amylbenzo-1,3-diphenol as the root and stem of Astragalus membranaceus.Besides,the mycelium also contained the same volatile component erucamide as the root and stem of Astragalus membranaceus.The endophytic fungus HHQ14 was a secondary metabolite producing bacterium of host plant Astragalus membranaceus,and contained a variety of volatile components with wide application and medical value.The above results lay a foundation for the comprehensive development and application of secondary metabolites.