青稞种质资源对大麦黄矮病毒的抗性鉴定和生理分析

Resistance Identification of Hulless Barley Germplasm Resources to Barley Yellow Dwarf Virus and Physiological Analysis

为明确青稞种质资源对大麦黄矮病毒(BYDV,barley yellow dwarf virus)的抗性水平,本研究采用田间人工接种法,对245份青稞材料进行了连续3年抗病性鉴定。供试材料中仅有08-1280表现为高抗黄矮病,6份材料(ZYM1289、北青6号、ZDM4409、甘青2号、藏青3000、ZYM1853)表现为抗病,中抗、感病、高感材料分别有47份、173份、18份。分子标记检测结果显示08-1280携带Yd2抗性基因。为了解大麦黄矮病毒侵染对青稞抗性生理指标的影响,分析了接种病毒后高抗材料08-1280、高感材料康青3号中总酚、脯氨酸、可溶性糖和可溶性蛋白含量的变化差异。接种后10 d,高抗材料总酚、脯氨酸含量增加幅度显著高于高感材料。接种后30 d时,高抗材料总酚含量增加幅度高于高感材料,可溶性糖含量增加幅度显著低于高感材料;与对照组相比,高抗材料可溶性蛋白显著下降,高感材料显著上升。本研究为青稞抗病品种培育和抗性机制研究提供了优异资源和理论参考。

To evaluate the resistance of hulless barley to barley yellow dwarf virus(BYDV),245 hulless barley genotypes were tested by inoculation with viruliferous aphids under field conditions in three calendar years.The results showed that the genotype 08-1280 was highly resistant to BYDV,and six genotypes(ZYM1289,Beiqing 6,ZDM4409,Ganqing 2,Zangqing 3000,ZYM1853)were resistant.47,173 and 18 genotypes showed moderately resistance,susceptibility and highly susceptibility to BYDV,respectively.The genotype 08-1280 was found carrying the resistance gene Yd2 based on genotyping using molecular marker.Moreover,to gain a better understanding of physiological mechanism of hulless barley against BYDV infection,the differences in total phenol,proline,soluble sugar and soluble protein content between resistant genotype 08-1280 and susceptible genotype Kangqing 3 were investigated.At 10 days after inoculation,the contents of total phenol and proline were elevated in 08-1280,higher than that of Kangqing 3.At 30 days after inoculation,a higher increase of total phenol in resistant genotype,if compared to that of susceptible genotype,was detected,while an opposite result for the soluble sugar was observed;the soluble protein decreased dramatically in 08-1280,whereas increased in Kangqing 3.Collectively,this study provided elite germplasm resources and theoretical reference in breeding of resistant varieties and analysis of resistant mechanisms in hulless barley.