利用分子标记筛选马铃薯抗孢囊线虫资源

Molecular Markers Assisted Identification of Potato Resources Resistant to Cyst Nematode

马铃薯孢囊线虫(PCN,potato cyst nematode)是马铃薯中重要的检疫性病害,可造成马铃薯减产80%。近年来,据报道西南地区多个马铃薯主产区发现马铃薯金线虫(Globodera rostochiensis)。为了我国马铃薯生产安全,筛选马铃薯孢囊抗性种质资源、选育抗性品种迫在眉睫。本研究利用前人报道的抗性分子标记N146、N195、57R、TG689、Gro1-4-1和Gpa2-2,对云南省农业科学院保存的875份马铃薯种质资源进行马铃薯孢囊线虫抗性标记筛选。筛选到211份资源含有马铃薯金线虫抗性基因H1分子标记(N146、N195、57R和TG689),其中114份资源中同时含有4个分子标记;7份资源含有马铃薯金线虫抗性基因Gro1-4分子标记Gro1-4-1;237份资源中含有马铃薯白线虫抗性基因Gpa2分子标记Gpa2-2。本研究结果将为马铃薯孢囊线虫抗性育种提供技术支撑和材料基础。

Potato cyst nematode(PCN)is an important quarantine disease and its infection can reduce the potato yield up to 80%.In recent years,the potato golden nematode(Globodera rostochiensis)was reported in several major producing areas in Southwest of China.In order to ensure the safety of potato production in China,it is urgent to identify germplasm resources with cyst resistance and breed for resistant varieties.Using the molecular markers N146,N195,57R,TG689,Gro1-4-1 and Gpa2-2 from earlier studies,in this study 875 potato germplasm accessions preserved by Yunnan Academy of Agricultural Sciences were genotyped for resistance genes to potato cyst nematode.211 accessions were detected with H1 molecular markers(N146,N195,57R and TG689)amplifying the resistance gene to potato cyst nematodes,of which 114 accessions contained four molecular markers at the same time.Seven accessions were identified carrying the resistance gene Gro1-4,while 237 resources were found with Gpa2-2 resistance gene Gpa2.Collectively,this study provided technical support and material basis for the breeding of potato cyst nematode resistance.