医务人员及家属人乳头瘤病毒自采样检测接受度调查及检测性能评价

Investigation of Acceptability of Human Papillomavirus Self-sampling Test in Medical Staff and Family Members and Evaluation of Detection Performance

目的:调查人乳头瘤病毒(HPV)自采样检测在医护人员和其家属、朋友中的接受和使用状况,评价其检测性能,探讨其在扩大子宫颈癌筛查覆盖率方面的应用前景。方法:选取2021年11月22—26日武警北京市总队医院医护人员和其家属、朋友130名为研究对象。对各科室医护人员进行HPV自采样宣教,自采样样本在检验与病理科同时采用流式荧光HPV27全分型试剂和荧光定量5+9高危HPV检测试剂完成HPV检测,比较使用自采样器妇女的基本信息,自采样样本HPV检测情况及自采样阳性受试者医院复查和阴道镜随访结果。结果:参与问卷调查的128名妇女中93.0%认为操作“方便”,96.9%表明未来“愿意”再次接受自采样。130名参与自采样的受试者年龄为26~75岁,HPV自采样检测阳性率为14.6%(19/130)。两种方法学对子宫颈癌高危14种HPV型别检测的一致性为100.00%,阳性受试者医院HPV检测的符合率为94.7%。阳性受试者1个月内阴道镜随访率为74%,3个月为随访率95%。19名自采样阳性患者阴道镜检查为高级别病变且组织学检测为CIN1以上的有6名。结论:HPV自采样器使用安全方便,检测准确,HPV自采样可提高妇女对HPV筛查的接受性和跟进检查的意愿。

Objective:To investigate the acceptance and use of human papillomavirus(HPV)self-sampling test among medical staffs,their families and friends,evaluate its performance,and explore its application prospects in expanding the coverage of cervical cancer screening.Methods:One hundred and thirty medical staff,their families and friends from Beijing Armed Police Corps Hospital from November 22,2021 to November 26,2021 were selected as the study subjects.HPV self-sampling education was carried out for medical staff in all departments.The self-sampled samples were tested by flow fluorescence HPV27 genotyping reagent and fluorescence quantitative 5+9 high-risk HPV detection reagent at the same time in the examination and pathology departments.The basic information of the women using the self-sampler,the HPV detection of the self-sampled samples and the results of the hospital re-examination and colposcopy follow-up of the self-sampled positive subjects were compared.Results:Of the 128 women who participated in the questionnaire,93.0%considered the operation"convenient"and 96.9%indicated that they were"willing"to accept self-sampling again in the future.The 130 participants who participated in the self-sampling were aged 26-75 years,and the positive rate of HPV self-sampling was 14.6%(19/130).The consistency of the two methods for detection of 14 high-risk HPV types of cervical cancer was 100.00%,and the coincidence rate of hospital HPV detection of positive subjects was 94.7%.The colposcopy follow-up rate of positive subjects within one month was 74%,and the follow-up rate of three months was 95%.Six of the 19 self sampling positive patients with Colposcopy showed high grade lesions and histologic detection of CIN1 or above.Conclusion:HPV self-sampler is safe,easy to use and accurate to detect.HPV self-sampling can improve women's acceptance of HPV screening and their willingness to follow up.