60 g/L乙基多杀菌素悬浮剂的高效液相色谱分析方法

Analysis of spinetoram 60 g/L SC by HPLC

[目的]采用HPLC法测定60 g/L乙基多杀菌素悬浮剂中有效成分含量。[方法]使用Poroshell 120 EC-C18色谱柱,检测波长245 nm;乙腈-0.05%乙酸铵水溶液(体积比为98∶2)为流动相;流速1 mL/min;40 ℃的柱温;10 μL样本检测容量的条件下对待测样品中乙基多杀菌素有效成分的含量进行检测。[结果]方法的线性相关系数为0.9998,标准偏差为0.03,变异系数为0.01%,平均回收率为98.61%。[结论]该方法操作简便,具有较高的准确度和精密度,适用于分析60 g/L乙基多杀菌素悬浮剂含量。

[Aims]To determine the content of active ingredient in spinetoram 60 g/L suspension concentrate by HPLC.[Methods]The determination of spinetoram active ingredient in the sample was performed on a Poroshell 120 EC-C18 column at 245 nm with a mobile phase of acetonitrile-0.05%ammonium acetate aqueous solution(98∶2,by vol),a flow rate of 1 mL/min,a column temperature of 40℃,and a sample volume of 10μL.[Results]The linear correlation coefficient of the method was 0.9998,the standard deviation was 0.03%,the coefficient of variation was 0.01%,and the mean recovery was 98.61%.[Conclusions]The method is easy to operate,has high accuracy and precision,and is suitable for the analysis of spinetoram 60 g/L suspension content determination.