益气升阳针法对慢性萎缩性胃炎大鼠胃黏膜GLi和COX-2蛋白表达

Influence of Replenishing Qi and Increasing Yang Acupuncture on Expressions of GLi and COX-2 Protein in Gastric Mucosa of Chronic Atrophic Gastritis Rats

目的观察应用益气升阳针法对慢性萎缩性胃炎大鼠胃黏膜GLi和COX-2蛋白表达。方法将60只健康SPF级Wistar大鼠采用随机数字表法依次分为正常组、模型组、西药维酶素组及针灸组各15只,除正常组外,其余各组大鼠均采用2%水杨酸钠和30%乙醇混合液1 mL/100 g混合灌胃,每天将100μg/mL MNNG作为饮用水自由饮用,同时结合饥饱失常法,建立慢性萎缩性胃炎大鼠模型。造模成功后,针灸组给予益气升阳针法治疗,西药维酶素组给予0.3 g/(kg·d)维酶素治疗,正常组及模型组每天给予生理盐水治疗,均连续治疗4周。治疗结束后,观察各组大鼠的一般情况、体质量及食量,各组胃黏膜GLi1、GLi2、GLi3、COX-2蛋白表达采用免疫组化染色观察,采用酶联免疫吸附法测定各组血清TNF-α、G-17、IL-1β及IL-4表达。结果各组体质量、食量相比;造模前,针灸组、西药维酶素组、模型组体质量、食量均低于正常组(P<0.05);干预后,正常组体质量、食量高于针灸组、西药维酶素组、模型组,针灸组体质量、食量高于西药维酶素组、模型组,西药维酶素组体质量、食量高于模型组(P<0.05)。正常组GLi1、GLi2、GLi3、COX-2蛋白表达及TNF-α、G-17、IL-1β及IL-4表达低于针灸组、西药维酶素组、模型组,针灸组GLi1、GLi2、GLi3、COX-2蛋白表达及TNF-α、G-17、IL-1β及IL-4表达低于西药维酶素组、模型组,西药维酶素组GLi1、GLi2、GLi3、COX-2蛋白表达及TNF-α、G-17、IL-1β及IL-4表达低于模型组(P<0.05)。结论针灸可增加慢性萎缩性胃炎大鼠体质量及食量,可能与降低GLi1、GLi2、GLi3、COX-2蛋白表达及TNF-α、G-17、IL-1β及IL-4表达有关,也是针灸防治慢性萎缩性胃炎的作用机制之一。

Objective To observe the effect of replenishing Qi and increasing Yang acupuncture on expressions of GLi and cy⁃clooxygenase-2(COX-2)protein in gastric mucosa of chronic atrophic gastritis rats.Methods Sixty healthy SPF Wistar rats were divided into normal group,model group,western medicine vitacoenzyme group and acupuncture group by random number ta⁃ble method,15 rats in each group except the normal group.Rats in the other groups were given 2%sodium salicylate and 30%ethanol mixed solution(1 mL/100 g)by gavage,N-Methyl-N'-nitro-N-nitrosoguanidine(MNNG)(100μg/mL)was used as the drinking water freely every day,and combined with the method of starvation and satiety,the rat model of chronic atrophic gastritis was established.After the model was successfully established,the acupuncture group was treated with acupuncture thera⁃py for replenishing Qi and increasing Yang,the western medicine group was treated with vitacoenzyme[0.3 g/(kg·d)],the nor⁃mal group and the model group were treated with normal saline every day,both of which were continuously treated for 4 week.Af⁃ter the treatment,the general condition,body weight and food intake of the rats in each group were observed.The expressions of GLi1,GLi2,GLi3 and COX-2 in the gastric mucosa of each group was observed by immunohistochemical staining,and the levels of serum tumor necrosis factor-α(TNF-α),gastrin(G-17),interleukin-1β(IL-1β)and interleukin-4(IL-4)of each group were determined by enzyme-linked immunosorbent method.Results After modeling,the body weights and appetites of the acupuncture group,western medicine vitacoenzyme group and model group were all lower than those of the normal group(P<0.05).After the intervention,the body weight and appetite of the normal group were higher than those of the acupuncture group,the western medicine vitacoenzyme group and the model group.The weight and appetite of the acupuncture group were higher than those of the western medicine vitacoenzyme group and the model group.The weight and appetite of the western medicine vi⁃tacoenzyme group were higher than those of the model group(P<0.05).The protein expressions of GLi1,GLi2,GLi3,COX-2 and the levels of TNF-α,G-17,IL-1βand IL-4 in the normal group were lower than those in the acupuncture group,the western medicine vitacoenzyme group and the model group.The protein expressions of GLi1,GLi2,GLi3,COX-2 and the levels of TNF-α,G-17,IL-1βand IL-4 of the acupuncture group were lower than those of the western medicine vitacoenzyme group and model group.The protein expressions of GLi1,GLi2,GLi3,COX-2 and the levels of TNF-α,G-17,IL-1βand IL-4 of the western medicine vitacoenzyme group were lower than those of the model group(P<0.05).Conclusion Acupunc⁃ture can increase the body weight and food intake in rats with chronic atrophic gastritis,which may be related to the decrease of expressions of GLi1,GLi2,GLi3 and COX-2 protein and the levels of TNF-α,G-17,IL-1βand IL-4.It is also one of the mechanisms of acupuncture in the prevention and treatment of chronic atrophic gastritis.