GMA接枝海藻酸钠光交联协同金属配位互穿网络凝胶的释药性能

Drug release properties of GMA-grafted sodium alginate photo-crosslinking with metal coordination interpenetrating network gel

用甲基丙烯酸缩水甘油酯(GMA)对海藻酸钠(Na-Alg)进行接枝改性得到了产物Alg-GMA,利用光引发自由基聚合协同二价阳离子(M^(2+),M代表Ca和Ba)配位交联作用,可调节海藻酸盐水凝胶(M-Alg-GMA)的结构与性能。采用FTIR、1HNMR、XPS、ICP和SEM对其进行了表征,并对其力学性能、溶胀性能、降解性能、体外释放、血液相容性及细胞毒性进行了测试。结果表明,Alg-GMA中出现了与GMA相关的氢信号。在光引发剂Irgacure 2959存在下,Alg-GMA经波长365 nm紫外光照射90 s即可快速形成Na-Alg-GMA水凝胶。Ca^(2+)和Ba^(2+)具有调节海藻酸盐水凝胶结构与性能的作用,这可能与Ca^(2+)的平面四方形构型dsp2轨道杂化方式和Ba^(2+)的正八面体构型d2sp3轨道杂化方式有关。Ca-Alg-GMA和Ba-Alg-GMA均具有肠道靶向释放药物能力,且均具有血液相容性好、细胞毒性低的特点。

Hydrogel alginate glycidyl methacrylate(Alg-GMA)was synthesized from sodium alginate(Na-Alg)was grafted with GMA,and underwent photoinitiated radical polymerization and bivalent cationic(M^(2+),M represents Ca or Ba)coordination crosslinking for adjustment of structure and properties of alginate hydrogels(M-Alg-GMA).The hydrogels obtained were then characterized by FTIR,1HNMR,XPS,ICP and SEM,followed by analyses on their mechanical strength,swelling properties,degradation,in vitro release,hemocompatibility and cytotoxicity.The results showed that Alg-GMA presented hydrogen signal associated with GMA.In the presence of photoinitiator Irgacure 2959,Alg-GMA could form hydrogel rapidly when irradiated by ultraviolet light with wavelength of 365 nm for 90 s.Furthermore,the structure and property regulation via Ca^(2+)and Ba^(2+)could be attributed to the dsp2 hybridization mode of Ca^(2+)in the planar quadrate configuration and d2sp3 hybridization mode of Ba^(2+)in the regular octahedral configuration.Both Ca-Alg-GMA and Ba-Alg-GMA exhibited intestinal targeted drug release,good blood compatibility and low cytotoxicity.