野大麦高效组培快繁及农杆菌介导的愈伤侵染体系建立

Establishment of Highly Efficient Tissue Culture and Agrobacterium-mediated Callus Infection Systems for Hordeum brevisubulatum

野大麦(Hordeum brevisubulatum)为禾本科大麦属多年生草本植物,具有较强的抗寒和耐盐碱能力,是挖掘抗逆基因的优良种质资源。但目前尚未见野大麦遗传转化体系的报道。该研究以蒙农1号杂交野大麦成熟胚为外植体诱导愈伤组织,建立了野大麦高效组培快繁体系,分化率达70%,快繁系数为35。在此基础上,利用根癌农杆菌(Agrobacterium tumefaciens)EHA105菌株侵染筛选获得的高质量愈伤系YZ101,通过优化侵染条件使侵染率接近30%。该体系的建立为野大麦功能基因组研究与分子设计育种奠定了基础。

Hordeum brevisubulatum is a perennial grass belonging to the genus Hordeum in the Poaceae with strong cold and salt-alkali tolerance,which is an excellent germplasm resource for mining stress resistance genes.However,there are few reports on the genetic transformation system in H.brevisubulatum.In this study,the mature embryos in wild barley(Mengnong 1 hybrid)seeds were employed as explants to induce high quality embryogenic calli.A highly efficient tissue culture system was established for Mengnong 1 hybrid wild barley with approximate 70%of differentiation rate and the propagation coefficient was 35.Furthermore,Agrobacterium tumefaciens strain EHA105 was used to infect the wild barley callus line YZ101,and the infection efficiency was approximate 30%with optimized infection conditions.This study will facilitate gene function deciphering and developing novel germplasm in H.brevisubulatum through molecular design breeding.