磁控溅射纳米银含量对钛种植体抗菌性的影响

Effect of Magnetron Sputtering Nano⁃Ag Content on Antibacterial Properties of Titanium Implants

纯钛因具有优良的生物相容性,被广泛应用于口腔种植相关领域,但其本身并不具备抗菌性能,为改善钛种植体表面的抗菌性,采用磁控溅射法在钛种植体表面制备Ti-Ag纳米复合涂层,研究了涂层中纳米Ag含量对具核梭杆菌(Fusobacterium nucleatum,Fn)抗菌性能的影响。分析了样品的表面形貌、粗糙度和水接触角;对Ag^(+)释放进行了检测;采用CCK-8法检测材料的细胞毒性;将各组样品与具核梭杆菌共培养,检测材料的抗菌性能。结果表明,载Ag复合涂层成功沉积在Ti片表面,并且随着纳米Ag含量的增加,样品的表面粗糙度和水接触角均增大。该Ti-Ag纳米复合涂层对小鼠L929细胞未表现出细胞毒性,符合生物安全标准。抗菌实验结果表明,随着纳米Ag含量的增加,Ag^(+)释放量增加,涂层的抗菌效果也增强。可见,Ti-Ag纳米复合涂层可有效抑制Fn的生长,有望提高钛种植体的抗菌性能,为其临床运用奠定了实验基础。

Pure titanium is widely used in oral implant-related fields because of its excellent biocompatibility,but it does not have antibacterial properties by itself.In order to improve the antibacterial properties of titanium implants surfaces,this paper used magnetron sputtering to prepare Ti-Ag nanocomposite coating on the surface of titanium implants and investigated the effect of nano-Ag content in the coating on the antibacterial properties of Fusobacterium nucleatum(Fn).The surface morphology,roughness and water contact angle of the samples were analyzed;the Ag^(+)release was examined;the cytotoxicity of the materials was examined by the CCK-8 method;and each group of samples were co-cultured with Fusobacterium nucleatum to examine the antibacterial properties of the materials.The results showed that the Ag-loaded composite coating was successfully deposited on the Ti sheet surface,and the surface roughness and water contact angle of the samples increased with the increase of nano-Ag content.The Ti-Ag nanocomposite coating did not exhibit cytotoxicity to mouse L929 cells and met biosafety standards.The results of antibacterial experiments showed that with the increase of nano-Ag content,the Ag^(+)release increased and the antibacterial effect of the coating was enhanced.It is concluded that the Ti-Ag nanocomposite coating can effectively inhibit the growth of Fn,which is expected to improve the antibacterial performance of titanium implants and lay an experimental foundation for its clinical application.