连翘醇提物对肺癌NCI-H226细胞增殖、迁移及侵袭的影响

Effects of Forsythia suspensa ethanol extract on the proliferation, migration and invasion of lung cancercells NCI-H226

目的 探究连翘醇提物对肺癌NCI-H226细胞增殖、迁移、侵袭的影响。方法 以肺癌NCI-H226细胞为研究对象,将细胞分为对照组,连翘醇提物低、中、高浓度组(5、10、20 mg/mL),激活剂组[10 mg/mL连翘醇提物+0.5μmol/L核因子κB(NF-κB)信号通路激活剂PMA],抑制剂组(10 mg/mL连翘醇提物+10μmol/L NF-κB信号通路抑制剂BAY 11-7082),阳性对照组(20μg/mL顺铂),除对照组细胞不作干预外,其余各组细胞加入相应药物培养24 h。检测细胞增殖、迁移、侵袭情况,并计算增殖率、迁移率、侵袭细胞数;检测细胞中NF-κB p65、NF-κB抑制蛋白α(IκBα)、磷酸化NF-κB p65(p-NF-κB p65)、磷酸化IκBα(p-IκBα)蛋白表达水平。结果 与对照组比较,连翘醇提物各浓度组和阳性对照组细胞的增殖率、迁移率、侵袭细胞数以及细胞中p-IκBα、p-NF-κB p65蛋白表达水平均显著降低(P<0.05);与连翘醇提物中浓度组比较,抑制剂组细胞增殖率、迁移率、侵袭细胞数及细胞中上述蛋白表达水平均显著降低(P<0.05),而激活剂组均显著升高(P<0.05)。结论 连翘醇提物可抑制肺癌NCI-H226细胞增殖、迁移和侵袭,其作用机制可能与抑制NF-κB信号通路有关。

OBJECTIVE To investigate the effects of Forsythia suspensa ethanol extract on the proliferation,migration and invasion of lung cancer cells NCI-H226.METHODS As research objects,lung cancer cells NCI-H226 were divided into control group,F.suspensa ethanol extract low-,medium-and high-concentration groups(5,10,20 mg/mL),activator group[10 mg/mL F.suspensa ethanol extract+0.5μmol/L nuclear factor kappa B(NF-κB)signaling pathway activator PMA],inhibitor group(10 mg/mL F.suspensa ethanol extract+10μmol/L NF-κB signaling pathway inhibitor BAY 11-7082)and positive control group(20μg/mL cisplatin).Except for the control group of cells without intervention,all other groups of cells were cultured with corresponding drugs for 24 hours;the proliferation,migration and invasion of cells were all detected,and the proliferation rate,migration rate,and the number of invading cells were also calculated;protein expressions of NF-κB p65,NF-κB inhibitory proteinα(IκBα),phosphorylated NF-κB p65(p-NF-κB p65)and phosphorylated IκBα(p-IκBα)were determined.RESULTS Compared with control group,the proliferation rate,migration rate,and the number of invading cells as well as the protein expressions of p-IκBαand p-NF-κB p65 were decreased significantly in F.suspensa ethanol extract groups and positive control group(P<0.05).Compared with F.suspensa ethanol extract medium-concentration group,the proliferation rate,migration rate,and the number of invading cells as well as above protein expressions were all decreased significantly in inhibitor group(P<0.05),while those of activator group were increased significantly(P<0.05).CONCLUSIONS F.suspensa ethanol extract can inhibit the proliferation,migration and invasion of lung cancer cells NCI-H226,and the mechanism of which may be related to the inhibition of NF-κB signaling pathway.