幽门螺杆菌抗生素耐药检测方法的研究进展

Approaches for detection of antibiotic resistance of Helicobacter pylori:Recent research advances

幽门螺杆菌(Helicobacter pylori,H.pylori)感染是一种传染性疾病,影响大约全球一半人口,与多种疾病发生发展相关,严重危害人类公共卫生健康.抗生素在H.pylori根除治疗中占据重要地位,目前常用方案包含了1-3种抗生素.抗生素耐药是H.pylori根除失败的主要原因之一,通过检测H.pylori耐药情况可实现个体化治疗、降低抗生素耐药率及提高H.pylori根除率.H.pylori抗生素耐药检测方法主要包括传统的药敏试验和分子生物学方法,如聚合酶链式反应(polymerase chain reaction,PCR)及其相关技术、DNA测序、荧光原位杂交(fluorescence in situ hybridization,FISH)、基因芯片等.本文就目前H.pylori抗生素耐药检测方法的进展做一综述,以指导医生在临床实践中选择合适的耐药检测方法.

Helicobacter pylori(H.pylori)infection,a contagious disease,has affected approximately half of the global population.It is associated with the occurrence and development of many diseases,which seriously endangers human public health.Antibiotics play an important role in H.pylori eradication therapy,and the common regimens for H.pylori eradication contain one to three antibiotics.Antibiotic resistance is one of the main reasons for the failure of H.pylori eradication.Detection of antibiotic resistance can be helpful for individualized management,reduction of drug resistance,and improvement of H.pylori eradication.Methods to detect antibiotic resistance of H.pylori primarily consist of traditional drug sensitivity tests and molecular biology methods,such as polymerase chain reaction and its related techniques,DNA sequencing,fluorescence in situ hybridization,and gene chip.This paper reviews the recent advances in approaches for detection of antibiotic resistance of H.pylori.