妊娠期糖尿病视网膜病变患者血清miR-200c-3p、 miR-20b-5p表达水平及检测临床意义

Expression levels and clinical significance of serum miR-200c-3p and miR-20b-5p in patients with gestational diabetic retinopathy

目的:探讨妊娠期糖尿病视网膜病变(DR)患者血清miR-200c-3p、miR-20b-5p水平变化及检测临床意义。方法:选择妊娠期糖尿病孕妇80例(GDM组)和DR孕妇75例(DR组),正常妊娠期女性95例(对照组)。采用qRT-PCR法对受试者血清miR-200c-3p、miR-20b-5p水平进行测定,采用全自动生化分析仪对受试者低密度脂胆固醇(LDL-C)、三酰甘油(TG)、总胆固醇(TC)进行测定,采用血糖检测仪对受试者空腹血糖(FPG)、餐后2 h血糖(2 hPG)进行测定。结果:GDM组、DR组患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于对照组(均P<0.05);DR组患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于GDM组(均P<0.05);随着病情严重程度的增加,DR组患者miR-200c-3p、miR-20b-5p水平显著升高(均P<0.05);预后不良组DR患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于预后良好组(均P<0.05);多因素Logistic回归分析显示血清miR-200c-3p、miR-20b-5p水平升高为DR患者发生预后不良的危险因素;ROC曲线结果显示,血清miR-200c-3p、miR-20b-5p、两者联合诊断DR患者不良预后曲线下面积(AUC)为0.927(95%CI:0.843~0.974)、0.848(95%CI:0.746~0.920)、0.985(95%CI:0.924~0.999)。结论:DR患者血清miR-200c-3p、miR-20b-5p水平升高,对DR不良预后具有一定的预测价值。

Objective:To explore the levels and clinical significance of serum miR-200c-3p and miR-20b-5p in patients with gestational diabetic retinopathy(DR).Methods:A total of 80 pregnant women with gestational diabetes mellitus(GDM group),75 pregnant women with DR(DR group)and 95 women with normal pregnancy(control group)were selected.The serum levels of miR-200c-3p and miR-20b-5p were measured by qRT-PCR.Low-density lipid cholesterol(LDL-C),triglyceride(TG)and total cholesterol(TC)were measured by automatic biochemical analyzer.Fasting plasma glucose(FPG)and 2-hour postprandial plasma glucose(2 hPG)were measured by blood glucose detector.Results:Compared with the control group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values in the GDM and DR groups were significantly increased(all P<0.05).Compared with the GDM group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values in the DR group were significantly increased(all P<0.05).As the severity of the disease increased,the levels of miR-200c-3p and miR-20b-5p in DR patients increased significantly(all P<0.05).Compared with the good prognosis group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values of DR patients in the poor prognosis group were significantly increased(all P<0.05).Multivariate Logistic regression analysis showed that elevated serum miR-200c-3p and miR-20b-5p levels were risk factors for poor prognosis in patients with DR.ROC curve results showed that the AUC of serum miR-200c-3p,miR-20b-5p,and the combined diagnosis for poor prognosis in patients with DR was 0.927(95%CI:0.843 to 0.974),0.848(95%CI:0.746 to 0.920),0.985(95%CI:0.924 to 0.999)respectively.Conclusion:The serum miR-200c-3p and miR-20b-5p levels of DR patients are elevated,which has certain diagnostic value for the poor prognosis of DR.