表面等离子共振生物传感器研究进展

Review of Biosensors Based on Surface Plasmon Resonance

表面等离子共振(SPR)现象因其对表面折射率变化的敏感而受到广泛关注,相应的SPR传感器以其独特的无标记、高灵敏度和快速检测的优势,在生物标志物检测、食品过敏原筛查和环境监测等领域具有广阔的应用潜力。本文介绍了SPR生物传感器的3种主要结构类型:棱镜耦合结构、光栅耦合结构、光纤耦合结构;着重研究了3种结构的检测原理、典型结构等传感特性及其进展;重点论述了SPR生物传感技术中生物功能化的研究现状和技术难题以及不同材料表面特性的SPR传感器;分析了目前SPR生物传感实际应用遇到的问题以及探讨了未来的研究方向;最后,结合实际情况,从结构和材料等方面展望了新型生物传感器的发展趋势。

The surface plasmon resonance(SPR)phenomenon has attracted considerable attention because of its sensitivity to the changes in the surface refractive index of materials.Sensors based on SPR have broad application potential in the fields of biomarker detection,food allergen screening,and environmental monitoring as they have advantages such as unmarked,high sensitivity and rapid detection.We review SPR biosensors based on three types of structures,namely prism coupling,grating coupling,and fiber coupling structures.The detection principle,typical structures and other sensing characteristics of the three structures and the advancements in their development are emphatically studied.Furthermore,the research status and technical problems related to biological functionalization in SPR biosensor technology are discussed.SPR sensors with different material surface characteristics are also discussed.The problems encountered in the practical application of SPR biosensors are analyzed,and the future research directions are presented.Finally,the prospects for the development trend of new biosensors are discussed based on the aspects of the structure and materials used.