冻融期刺参养殖池塘水体菌群结构与功能特征

The structural and functional characteristics of bacterial community in Apostichopus japonicus culture ponds during freeze-thaw period

为解析冻融期刺参养殖池塘水体菌群结构与功能特征,本研究以中国北方典型刺参养殖池塘为对象,利用16S rRNA测序技术,开展冰封期和融冰期刺参养殖池塘水体菌群结构与功能共性及特异性研究。结果表明:冻融期优势菌群均隶属于变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);冰封期水体特异性菌群为梭杆菌门(Fusobacteria)和酸杆菌门(Acidobacteria),融冰期特异性菌群则主要隶属于放线菌门(Actinobacteria);基于KEGG数据库分析可知,冰封期和融冰期水体菌群代谢通路具有显著性差异(P<0.05),差异代谢通路主要表现在新陈代谢方面,以能量代谢、其他次生代谢物的生物合成、核苷酸代谢为代表;冰封期和融冰期刺参养殖池塘水体菌群结构与功能存在显著性差异。研究结果为刺参养殖池塘精细化管理和刺参健康养殖提供科学依据。

The aim of this study was to clarify the structural and functional characteristics of bacterial community in Apostichopus japonicus culture ponds during freeze-thaw period.With a typical A.japonicus culture pond in northern China as the research object,we used 16S rRNA high-throughput sequencing to study the generality and specificity of structure and function of bacterial community in the water of A.japonicus culture ponds in freeze period and thaw period.The results showed that the dominant bacterial groups during freeze-thaw period were Proteobacteria,Bacte-roidetes,and Actinobacteria.The specific bacterial groups in the freeze period were composed of Fusobacteria and Acidobacteria,while that in the thaw period was mainly composed of Actinobacteria.Based on the analysis of KEGG database,there were significant differences in the metabolic pathways of bacterial communities between freeze period and thaw period.The differential metabolic pathways were mainly manifested in metabolism,represen-ted by energy metabolism,biosynthesis of other secondary metabolites,and nucleotide metabolism.There were sig-nificant differences in the structure and function of bacterial community in water of Apostichopus japonicus culture ponds during freeze period and thaw period.Our results provide a theoretical basis for fine management of A.japoni-cus culture ponds and healthy culture of A.japonicus.